Purpose(Empty Backbone) Donor empty vector with floxed puromycin resistance gene expression cassette
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68407||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerFrederick National Lab
- Backbone size (bp) 4800
Modifications to backbonePL552 donor plasmid vector containing a floxed PGK-puromycin expression cassette was constructed by replacing the neomycin gene in the PL452 (Frederick National Lab) with a puromycin gene.
Vector typeMammalian Expression, Cre/Lox
- Promoter mouse PGK promoter
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer agctatgaccatgattacg
- 3′ sequencing primer aaacgacggccagtgaattg (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PL552 was a gift from Su-Chun Zhang (Addgene plasmid # 68407 ; http://n2t.net/addgene:68407 ; RRID:Addgene_68407)
For your References section:Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. 10.1016/j.stem.2015.06.001 PubMed 26145478