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pMo130-TelR
(Plasmid #50799)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50799 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMo130
  • Backbone manufacturer
    Martin Voskuil
  • Backbone size w/o insert (bp) 6127
  • Total vector size (bp) 9392
  • Vector type
    Suicide vector
  • Selectable markers
    Tellurite

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Also resistant to Tellurite
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tellurite-resistance cassette
  • Alt name
    TelR
  • Species
    RK2 plasmid
  • Insert Size (bp)
    3266
  • Promoter PCs12

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer 5'-CGGGTACCGAGCTCGAATTA-3'
  • 3′ sequencing primer 5'-CCGGGTACCGAGCTCGAATT-3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gift from Dr Tung T. Hoang, Department of Microbiology, University of Hawaii at Manoa, 2358 The Mall-Snyder 310, Honolulu HI 96822
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMo130-TelR was a gift from Kim Lee Chua (Addgene plasmid # 50799 ; http://n2t.net/addgene:50799 ; RRID:Addgene_50799)
  • For your References section:

    A method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii. Amin IM, Richmond GE, Sen P, Koh TH, Piddock LJ, Chua KL. BMC Microbiol. 2013 Jul 13;13:158. doi: 10.1186/1471-2180-13-158. 10.1186/1471-2180-13-158 PubMed 23848834