pMo130
(Plasmid #27388)

• Purpose: (Empty Backbone) Burkholderia allelic exchange suicide vector pMo130, ref EU862243
• Depositing Lab: Martin Voskuil
• Publication: Hamad et al Gene. 2009 Feb 1. 430(1-2):123-31.

Backbone

• Vector backbone: pUC19
• Backbone size (bp): 6127
• Vector type: Burkholderia allelic exchange suicide vector pMo130

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): JM109
• Growth instructions: Use JM109 bacterial strain; Grow at 30C
• Copy number: High Copy

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Ref: EU862243
MCS-1: ApaI, NheI, BglII, SmaI, HindIII
MCS-2 NotI, PstI, BamHI, XbaI, SphI

Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp# 5006, a 5 nucleotide deletion at bp# 5981-5985 and a region from bp#1010-1060 with several mismatches/deletions, when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing scientist, these differences do not affect the function of the plasmid.