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(Plasmid #27389)


Item Catalog # Description Quantity Price (USD)
Plasmid 27389 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 7108
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Use JM109 bacterial strain; Grow at 30C
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
    E. coli
  • Mutation
    A replicative vector for gene expression in Burkholderia
  • GenBank ID

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MCS1: ApaI, NheI, BglII, SmaI, HindIII (not destroyed)
  • 3′ cloning site MCS2: PstI, BamHI, XbaI, SphI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp# 5987 and a 5 nucleotide deletion at bp# 6962-6966, when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing scientist, these differences do not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMo168 was a gift from Martin Voskuil (Addgene plasmid # 27389 ; ; RRID:Addgene_27389)
  • For your References section:

    An allelic exchange system for compliant genetic manipulation of the select agents Burkholderia pseudomallei and Burkholderia mallei. Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI. Gene. 2009 Feb 1. 430(1-2):123-31. 10.1016/j.gene.2008.10.011 PubMed 19010402