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PT3.5/CMV-EGFRvIII
(Plasmid #20280)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20280 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    colE1/ampR
  • Backbone manufacturer
    Ohlfest Lab
  • Backbone size w/o insert (bp) 5481
  • Vector type
    Transposon

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EGFRvII
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2832
  • Mutation
    See depositor comments below.
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, HER1, NISBD2, PIG61, mENA)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Addgene NGS results identified a E423G variant in EGFRvIII compared to the NCBI reference (NP_001333870.1). We do not know if this mutation affects function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PT3.5/CMV-EGFRvIII was a gift from John Ohlfest (Addgene plasmid # 20280 ; http://n2t.net/addgene:20280 ; RRID:Addgene_20280)
  • For your References section:

    De novo induction of genetically engineered brain tumors in mice using plasmid DNA. Wiesner SM, Decker SA, Larson JD, Ericson K, Forster C, Gallardo JL, Long C, Demorest ZL, Zamora EA, Low WC, SantaCruz K, Largaespada DA, Ohlfest JR. Cancer Res. 2009 Jan 15. 69(2):431-9. 10.1158/0008-5472.CAN-08-1800 PubMed 19147555