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Xiaokun Shu Lab Plasmids


The Xiaokun Shu Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Yu D, Gustafson WC, Han C, Lafaye C, Noirclerc-Savoye M, Ge WP, Thayer DA, Huang H, Kornberg TB, Royant A, Jan LY, Jan YN, Weiss WA, Shu X Nat Commun. 2014 May 15;5:3626. doi: 10.1038/ncomms4626.
A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K, Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, Davidson MW, Shu X Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 2015 Jun 22.
Rationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo. To TL, Piggott BJ, Makhijani K, Yu D, Jan YN, Shu X Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3338-43. doi: 10.1073/pnas.1502857112. Epub 2015 Mar 2.
Rational design of a monomeric and photostable far-red fluorescent protein for fluorescence imaging in vivo. Yu D, Dong Z, Gustafson WC, Ruiz-Gonzalez R, Signor L, Marzocca F, Borel F, Klassen MP, Makhijani K, Royant A, Jan YN, Weiss WA, Guo S, Shu X Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015 Dec 31.
Rational Design of a GFP-Based Fluorogenic Caspase Reporter for Imaging Apoptosis In Vivo. To TL, Schepis A, Ruiz-Gonzalez R, Zhang Q, Yu D, Dong Z, Coughlin SR, Shu X Cell Chem Biol. 2016 Jul 21;23(7):875-82. doi: 10.1016/j.chembiol.2016.06.007.
Precision Optogenetic Tool for Selective Single- and Multiple-Cell Ablation in a Live Animal Model System. Makhijani K, To TL, Ruiz-Gonzalez R, Lafaye C, Royant A, Shu X Cell Chem Biol. 2017 Jan 19;24(1):110-119. doi: 10.1016/j.chembiol.2016.12.010. Epub 2017 Jan 5.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
54620mIFP-N1Mammalian ExpressionA naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 2015 Jun 22. Localization: N1 Cloning Vector, Excitation: 683, Emission: 704 mIFP-N1
  • Tag / Fusion Protein
    • mIFP (C terminal on backbone)
  • Add to Cart
    54784IFP2.0-C1 Mammalian ExpressionAn improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nat Commun. 2014 May 15;5:3626. doi: 10.1038/ncomms4626. Localization: C1 Cloning Vector, Excitation: 684, Emission: 708 IFP2.0-C1
  • Tag / Fusion Protein
    • IFP2.0 (N terminal on backbone)
  • Add to Cart
    54785IFP2.0-N1Mammalian ExpressionAn improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nat Commun. 2014 May 15;5:3626. doi: 10.1038/ncomms4626. Localization: N1 Cloning Vector, Excitation: 684, Emission: 708 IFP2.0-N1
  • Tag / Fusion Protein
    • IFP2.0 (C terminal on backbone)
  • Add to Cart
    54819mIFP12-C1 Mammalian ExpressionA naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 2015 Jun 22. Localization: C1 Cloning Vector, Excitation: 683, Emission: 704 mIFP12-C1
  • Tag / Fusion Protein
    • mIFP12 (N terminal on backbone)
  • Add to Cart
    54820mIFP24-C1 Mammalian ExpressionA naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 2015 Jun 22. Localization: C1 Cloning Vector, Excitation: 683, Emission: 704 mIFP24-C1
  • Tag / Fusion Protein
    • mIFP24 (N terminal on backbone)
  • Add to Cart
    59427pLenti CMV-IFP2.0 T2A HO1IFP2.0T2AHO1 IRES EGFPLentiviralAn improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nat Commun. 2014 May 15;5:3626. doi: 10.1038/ncomms4626. Express infrared fluorescent protein IFP2.0 plus HO1 enzyme pLenti CMV/TO Puro DEST (670-1 Add to Cart
    64276pcDNA3.1 TEV (full-length)Tobacco etch virus (TEV) protease (Synthetic)Mammalian ExpressionRationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3338-43. doi: 10.1073/pnas.1502857112. Epub 2015 Mar 2. Expresses tobacco etch virus protease (TEV) pcDNA3.1 Add to Cart
    64277pcDNA3.1 iTEVInfrared fluorescent tobacco etch virus protease sensor (iTEV) (Synthetic)Mammalian ExpressionRationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3338-43. doi: 10.1073/pnas.1502857112. Epub 2015 Mar 2. Expresses infrared fluorescent tobacco etch virus protease sensor (iTEV) pcDNA3.1 Add to Cart
    64278pcDNA3.1 iCasper T2A HO1Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (Synthetic)Mammalian ExpressionRationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3338-43. doi: 10.1073/pnas.1502857112. Epub 2015 Mar 2. Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1 pcDNA3.1 HMOX1 HMOX1D, HO-1, HSP32, bK286B10 Add to Cart
    72433pCS2+_Myr-mIFPMry-mIFPRational design of a monomeric and photostable far-red fluorescent protein for fluorescence imaging in vivo. Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015 Dec 31. expressing of Myr-mIFP in Zebrafish pcS2+ Add to Cart
    72434iBlueberry T2A mCherryiBlueberry T2A mCherryMammalian ExpressionRational design of a monomeric and photostable far-red fluorescent protein for fluorescence imaging in vivo. Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015 Dec 31. Expressing of iBlueberry and mCherry in mammalian cells pcDNA3.1 Add to Cart
    72441pACU2_CD4-mIFP T2A HO1CD4-mIFP T2A HO1Insect ExpressionA naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 2015 Jun 22. Expressing UAS-CD4-mIFP T2A HO1 in drosophila pACU2 Add to Cart
    72443pLenti CMV_mIFP IRES EGFPmIFP T2A HO1 IRES EGFP (Other)Mammalian Expression, LentiviralA naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 2015 Jun 22. Expressing mIFP and EGFP in mammalian cells pLentiCMV dest#2 Add to Cart
    73176pcDNA3.1_LifeAct-iBlueberryLifeAct-iBlueberryMammalian ExpressionRational design of a monomeric and photostable far-red fluorescent protein for fluorescence imaging in vivo. Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015 Dec 31. Expressing iBlueberry and mCherry in mammalian cells pN1 Add to Cart
    81241ZipGFP-Casp3ZipGFP with caspase cleavage sequence T2A mCherry (Synthetic)Mammalian ExpressionRational Design of a GFP-Based Fluorogenic Caspase Reporter for Imaging Apoptosis In Vivo. Cell Chem Biol. 2016 Jul 21;23(7):875-82. doi: 10.1016/j.chembiol.2016.06.007. A ZipGFP-based Caspase-3 protease reporter T2A mCherry pcDNA3.1 Add to Cart
    81242ZipGFP1-10_TEVzipped GFP11 with TEV cleavage sequence T2A mCherry (Synthetic)Mammalian ExpressionRational Design of a GFP-Based Fluorogenic Caspase Reporter for Imaging Apoptosis In Vivo. Cell Chem Biol. 2016 Jul 21;23(7):875-82. doi: 10.1016/j.chembiol.2016.06.007. Zipped GFP1-10 with TEV cleavage sequence T2A mCherry pcDNA3.1 Add to Cart
    81243ZipGFP11_TEVzipped GFP11 with TEV cleavage sequence (Synthetic)Mammalian ExpressionRational Design of a GFP-Based Fluorogenic Caspase Reporter for Imaging Apoptosis In Vivo. Cell Chem Biol. 2016 Jul 21;23(7):875-82. doi: 10.1016/j.chembiol.2016.06.007. Zipped GFP11 with TEV cleavage sequence pcDNA3.1 Add to Cart
    87410pcDNA3.1_miniSOG2 T2A H2B-EGFPmini singlet oxygen generator 2Mammalian ExpressionPrecision Optogenetic Tool for Selective Single- and Multiple-Cell Ablation in a Live Animal Model System. Cell Chem Biol. 2017 Jan 19;24(1):110-119. doi: 10.1016/j.chembiol.2016.12.010. Epub 2017 Jan 5. Mammalian expression vector encoding Histone 2B GFP fusion and miniSOG2 pcDNA3.1 Add to Cart
    87411pIHEU_miniSOG2 T2A H3.3A-EGFPmini singlet oxygen generator 2Insect ExpressionPrecision Optogenetic Tool for Selective Single- and Multiple-Cell Ablation in a Live Animal Model System. Cell Chem Biol. 2017 Jan 19;24(1):110-119. doi: 10.1016/j.chembiol.2016.12.010. Epub 2017 Jan 5. UAS vector to express Histone 3.3A GFP fusion and miniSOG2 in Drosophila pIHEU Add to Cart