165,839 results

1. pLBH531_MBP-Cas14a1 expression

   Plasmid

   #112500

   Purpose

   Expresses MBP fused to Cas14a1

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. OaAEP1_C247A

   Plasmid

   #200307

   Purpose

   Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)

   Depositor

   Artur Krężel

   Article

   Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27

   Insert

   Asparaginyl endopeptidase from Oldenlandia affinis
   

   Use

   Tags

   Smt3

   Expression

   Bacterial

   Mutation

   Cysteine 247 to Alanine

   Promoter

   T7

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.23-GW

   Plasmid

   #60323

   Purpose

   This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-6p-2-EWSR1

   Plasmid

   #46384

   Depositor

   Heinz Gehring

   Article

   Pahlich et al Proteins. 2005 Oct 1;61(1):164-75.

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-EF1α-DIO-CFL-SN

   Plasmid

   #181740

   Purpose

   AAV vector for expressing CFL-SN

   Depositor

   Yasunori Hayashi

   Article

   Goto et al Science. 2021 Nov 12;374(6569):857-863.

   Insert

   CFL-SN
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHBintE

   Plasmid

   #48136

   Purpose

   Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2494

   Plasmid

   #142112

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZBTB18 (ZBTB18 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3TS-spRYCas9-ABE-UMax

   Plasmid

   #222139

   Purpose

   Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   TadA-nSpRYCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. FRB-mCherry-Giantin

   Plasmid

   #186575

   Purpose

   Expression of FRB-mCherry on Golgi membrane with Giantin transmembrane (residues 3131-3259)

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   Giantin (GOLGB1 Human)
   

   Use

   Tags

   FRB-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNatA (pACYCduet-naa10-naa15)

    Plasmid

    #72928

    Purpose

    Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Inserts

    naa10 (naa10 Fission Yeast, Synthetic)
    naa15 (naa15 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optomised for E.coli expression

    Promoter

    T7

    Available Since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PMP34-mCherry-EGFP-Tet-On

    Plasmid

    #109017

    Purpose

    Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    PMP34 (SLC25A17 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. BPK2014-AaCas12b

    Plasmid

    #121949

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Use

    Tags

    10xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF1906

    Plasmid

    #141883

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPROBE-GT

    Plasmid

    #37814

    Depositor

    Steven Lindow

    Article

    Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    Promotorless gfp reporter gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pUCmini-iCAP-PHP.B

    Plasmid

    #103002

    Purpose

    non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. mTFP1-N1

    Plasmid

    #54521

    Purpose

    Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Type

    Empty backbone

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTN4

    Plasmid

    #174212

    Purpose

    Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Inserts

    AtTIR1(F79G)::mRuby (TIR1 Nematode)
    GFP
    

    Use

    CRISPR and Cre/Lox

    Tags

    mRuby

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. coel::RFP

    Plasmid

    #8938

    Depositor

    Piali Sengupta

    Article

    Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

    Insert

    unc-122 promoter (unc-122 Nematode)
    

    Use

    Tags

    RFP

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Nov. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF1-Hs Cdk1/cyclin B1

    Plasmid

    #177330

    Purpose

    Co-expression of the human active Cdk1/cyclin B1 kinase complex

    Depositor

    Andreas Boland

    Article

    Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

    Inserts

    Cyclin-dependent kinase 1 (CDK1 Human)
    G2/mitotic-specific cyclin-B1 (CCNB1 Human)
    

    Use

    Tags

    2 x Strep-tag and 8 x His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. RNASEL gRNA (BRDN0001149212)

    Plasmid

    #77051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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