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Cas12f-GE ver4.1
(Plasmid #176544)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 176544 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    px458-GFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cas12f-GE ver4.1
  • Alt name
    Cas14a-GE ver4.1
  • Alt name
    Un1Cas12f1-GE ver4.1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1680
  • Promoter chicken β-actin

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Cas12f-GE ver4.1
  • Alt name
    Cas14a-GE ver4.1
  • Alt name
    MS2/MS3/MS4/MS5 sgRNA modifications
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    199
  • Promoter U6

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cas12f-GE ver4.1 was a gift from Yong-Sam Kim (Addgene plasmid # 176544 ; http://n2t.net/addgene:176544 ; RRID:Addgene_176544)
  • For your References section:

    Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus. Kim DY, Lee JM, Moon SB, Chin HJ, Park S, Lim Y, Kim D, Koo T, Ko JH, Kim YS. Nat Biotechnol. 2021 Sep 2. pii: 10.1038/s41587-021-01009-z. doi: 10.1038/s41587-021-01009-z. 10.1038/s41587-021-01009-z PubMed 34475560