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PurposeExpresses Cas12f-GE in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepx458-GFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCas12f-GE ver4.1
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Alt nameCas14a-GE ver4.1
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SpeciesH. sapiens (human)
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Insert Size (bp)1680
Gene/Insert 2
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Gene/Insert nameCas12f-GE ver4.1
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Alt nameCas14a-GE ver4.1
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SpeciesH. sapiens (human)
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Insert Size (bp)199
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cas12f-GE ver4.1 was a gift from Yong-Sam Kim (Addgene plasmid # 176544 ; http://n2t.net/addgene:176544 ; RRID:Addgene_176544) -
For your References section:
Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus. Kim DY, Lee JM, Moon SB, Chin HJ, Park S, Lim Y, Kim D, Koo T, Ko JH, Kim YS. Nat Biotechnol. 2021 Sep 2. pii: 10.1038/s41587-021-01009-z. doi: 10.1038/s41587-021-01009-z. 10.1038/s41587-021-01009-z PubMed 34475560