161,273 results

1. RMCE halo-flag DNA-PKcs

   Plasmid

   #233918

   Purpose

   Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

   Depositor

   Katheryn Meek

   Article

   Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

   Insert

   human DNA-PKcs (PRKDC Human)
   

   Use

   Tags

   flag-halo

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. HRE-doUnaG

   Plasmid

   #201713

   Purpose

   Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

   Depositor

   Friedemann Kiefer

   Article

   Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJ4M_hnRNPA2_FL_WT

   Plasmid

   #139109

   Purpose

   Express FL hnRNPA2 with a C-terminal MBP tag

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF3-microID

   Plasmid

   #172880

   Purpose

   expresses microID in mammalian cells with the tet-on or tet-off system

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   microID
   

   Use

   Luciferase and Retroviral; To be used in a tet-on…

   Tags

   myc

   Expression

   Mammalian

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   TRE-cmv

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MZB209

   Plasmid

   #228492

   Purpose

   BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)

   Depositor

   Richard Baker

   Insert

   BirA (birA )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. ITSN-mcherry-sspB

   Plasmid

   #85220

   Purpose

   Role of Cdc-42 selective GEF domain from ITSN in immune cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

   Inserts

   ITSN1(1225-1575) (ITSN1 Human)
   mcherry
   sspB
   

   Use

   Tags

   NA

   Expression

   Mammalian

   Mutation

   R73Q

   Promoter

   CMV

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOpen-MMLV_RT (mut H)

   Plasmid

   #165546

   Purpose

   Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.

   Depositor

   FreeGenes Project, Drew Endy, Jennifer Molloy

   Insert

   Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pVITRO-HPV18 L1L2

   Plasmid

   #52592

   Purpose

   Expresses HPV18 L1 and L2 in mammalian cells

   Depositor

   Richard Roden

   Article

   Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

   Inserts

   HPV18 L1
   HPV18 L2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAKlux2

   Plasmid

   #14080

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   Bacterial luciferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac, T3

   Available since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Syn-DIO-PinkyCaMP

    Plasmid

    #232857

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame)

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSin Capsid

    Plasmid

    #127693

    Purpose

    Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    Sindbis Capsid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pM-Cas12a

    Plasmid

    #196291

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Plant

    Mutation

    See depositor comments below

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-FGFR2-N549K

    Plasmid

    #116380

    Purpose

    Lentiviral expression of FGFR2 N549K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR2 (FGFR2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    N549K

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriEx4-Rac1-2G

    Plasmid

    #66110

    Purpose

    Second generation Rac1 FRET biosensor

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-F-del Casp9.IRES.GFP

    Plasmid

    #15567

    Depositor

    David Spencer

    Article

    Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

    Insert

    FKBP12(V36)-p30Caspase9 (FKBP1A Human)
    

    Use

    Retroviral

    Tags

    FKBP12 and HA epitope

    Expression

    Mammalian

    Mutation

    FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

    Promoter

    Available since

    Aug. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDB_345

    Plasmid

    #231113

    Purpose

    ABE activity-based selection cloning vector

    Depositor

    John Doench, David Root

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMVtight-UPRT-T2A-RFP-IRES-CD

    Plasmid

    #126677

    Purpose

    Doxycycline inducible expression of UPRT and CD along with reporter RFP

    Depositor

    Joan Massague

    Article

    Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

    Insert

    UPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    tight TRE

    Available since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRRL.PPT.SFFV.MCS.IRES.dTomato

    Plasmid

    #171174

    Purpose

    Lentiviral expression vector co-expressing dTomato

    Depositor

    Dirk Heckl

    Insert

    dTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Halo-Rab6A

    Plasmid

    #190171

    Purpose

    Expresses HaloTag-Rab6A in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    HaloTag-Rab6A
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSK-mU6-Cj-sgRNA-SV40-puro

    Plasmid

    #192128

    Purpose

    Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Cj-SgRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge