179,919 results
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Plasmid#128146PurposeTo express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEMDepositorTypeEmpty backboneTagsFlag-APEX2ExpressionMammalianAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only
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pDEST-CMV-3xFLAG-gateway-EGFP
Plasmid#122845PurposeN-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway CloningTags3xFLAG and EGFPExpressionMammalianPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)
Plasmid#107942PurposeMammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutionsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
Tags3x HA and NLS (nucleoplasmin)ExpressionMammalianMutationE174R, S542R, K548R, and N282APromoterCAGAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCM-CLYBL-hNIL
Plasmid#105841PurposeDonor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuronDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.GCaMP6m.WPRE.SV40 (AAV9)
Viral prep#100841-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC1-SypHer3s
Plasmid#108118PurposeRatiometric pH probe with enhanced brightness for mammalian cells expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSypHer3s
Tagsratiometric YFPExpressionMammalianPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-ERK-SPARK
Plasmid#106921PurposeExpress ERK-SPARK, an activity reporter of ERK in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERK-SPARK
ExpressionMammalianAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CamKII-ArchT-GFP (PV2527) (AAV5)
Viral prep#99039-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CamKII-ArchT-GFP (PV2527) (#99039). In addition to the viral particles, you will also receive purified pAAV-CamKII-ArchT-GFP (PV2527) plasmid DNA. CaMKII-driven ArchT-GFP expression for optogenetic inhibition.DepositorPromoterCaMKIITagsGFPAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Chronos-GFP (AAV Retrograde)
Viral prep#59170-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-Syn-Chronos-GFP (#59170). In addition to the viral particles, you will also receive purified pAAV-Syn-Chronos-GFP plasmid DNA. Chronos-GFP under the control of the Synapsin promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFPAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xnls-mScarlet-I
Plasmid#98816PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xNLS
TagsmScarlet-IExpressionMammalianPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFLP-hyg
Plasmid#87831PurposeExpresses FLP recombinase with hyg selection markerDepositorInserthyg
TagsNoneExpressionBacterialMutationNonePromoterheat-shock inducibleAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV pEF1a-DIO-FLPo-WPRE-hGHpA
Plasmid#87306PurposeAn AAV vector expresses FLPo in a Cre recombinase-dependent manner.DepositorHas serviceAAV1, AAV2, AAV5, AAV9, and AAV RetrogradeInsertCre-dependent mouse codon-optimized Flp recombinase.
UseAAVTagsN/AExpressionMammalianPromoterEF1aAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCSII-EF-miRFP670v1-hGem(1/110)
Plasmid#80006PurposehGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)DepositorAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-puro-CMV-VC3AI
Plasmid#78907PurposeIndicator of caspase-3-like protease activityDepositorInsertVC3AI
UseLentiviralTagsHA and MycMutationS320A, deletion of AA1-200PromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRC-APEX2
Plasmid#72558PurposeExpression of cytosolic APEX2 in bacteriaDepositorInsertAPEX2
TagsHis6ExpressionBacterialMutationA134P relative to APEXAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Flag-IDH1-R132H
Plasmid#62907PurposeExpresses R132 mutant IDH1 in mammalian cellsDepositorAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEvol-pAzFRS.1.t1
Plasmid#73547PurposetRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.1.t1
pAzFRS.1.t1
ExpressionBacterialAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ143
Plasmid#69295PurposeGolden Gate recipient and Gateway entry vector; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialPromoterT7Available sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3-Basic-IRES
Plasmid#64784PurposeLuciferase reporter vectorDepositorTypeEmpty backboneUseLuciferaseAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by