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pLCS109

Plasmid

#209330

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mTagBFP2 fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pATT-BsaI-Pmag-NmagHigh1

Plasmid

#202713

Purpose

Contains Magnet flanked by linkers and BsaI sites insert for transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

linker-pMag-RBS-nMagHigh1-linker

Use

Staging plasmid to make transposon insertion libr…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0733

Plasmid

#141676

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PROX2 (PROX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-180_AE

Plasmid

#148578

Purpose

Mammalian Expression of HsGIGYF2_1-180

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180 (GIGYF2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC34A2_STOP

Plasmid

#161254

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC34A2 (SLC34A2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC34A3_STOP

Plasmid

#161242

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC34A3 (SLC34A3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC20A2_STOP

Plasmid

#161188

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC20A2 (SLC20A2 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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tubulin-OeNL_pcDNA3

Plasmid

#89523

Purpose

Orange color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

b-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pME-Cas9

Plasmid

#63154

Purpose

Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Insert

Cas9

Use

CRISPR; Tol2 middle entry vector for gateway

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCCL-CellCycle

Plasmid

#132429

Purpose

Expressing Fucci cell cycle indicator in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

hCdt, hGeminin

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

Plasmid

#188375

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLIX403 RNaseH1

Plasmid

#237695

Purpose

Inducible lentiviral expression of RNaseH1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

RNaseH1 (RNASEH1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-tdTomato (AAV1)

Viral Prep

#128434-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-tdTomato (#128434). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-tdTomato plasmid DNA. EF1a-driven, Flp-dependent expression of tdTomato. These AAV preparations are suitable purity for injection into animals.

Depositor

Patricia Jensen

Article

Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

Promoter

EF1a

Tags

tdTomato (Flp-dependent)

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only
pLenti.CAG.H2B-Dendra2.W

Plasmid

#51005

Purpose

Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

Depositor

Rusty Lansford

Insert

H2B-Dendra2

Use

Lentiviral

Tags

histone2B

Expression

Mammalian

Promoter

CAG

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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pET-scFv-T

Plasmid

#67843

Purpose

pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

Depositor

Joanna Bereta

Article

Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

Insert

antibody 1D8 light kappa chain

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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12XCSL-d1EGFP

Plasmid

#47684

Purpose

Notch reporter

Depositor

Urban Lendahl

Article

Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

Insert

TP-1 promoter

Use

Notch reporter construct

Tags

d1EGFP

Mutation

hexamerized 50-bp EBNA2 response element of the T…

Promoter

NA

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pFW2500

Plasmid

#224213

Purpose

Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1 and recT

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

Plasmid

#225242

Purpose

Mammalian expression of iPASnFR-L in the cytosol

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP1

Plasmid

#22596

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP1 (USP1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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TRPML1-EGFP

Plasmid

#245039

Purpose

Expresses TRPML1 with C-terminus EGFP tag in mammalian cells

Depositor

Thomas Martin

Insert

TRPML1 (MCOLN1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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