165,843 results

1. pGL0_5 [pAL5000 ORI]

   Plasmid

   #198922

   Purpose

   Level 0 part

   Depositor

   Nili Ostrov, Charlie Gilbert, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pAL5000 ORI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-mDlx-ChR2-mCherry-Fishell-3

   Plasmid

   #83898

   Purpose

   ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   ChR2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mDlx

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe neo TGFBR3-HA

   Plasmid

   #83095

   Purpose

   Retroviral vector for constitutive TGFBR3 expression with C-terminal HA tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   TGFBR3 (TGFBR3 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

4. FH1-MGT#1-mVenus

   Plasmid

   #164094

   Purpose

   Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

   Insert

   MGT#1-mVenus
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiGuide Cherry

   Plasmid

   #170510

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Pavel Tolar

   Article

   Newman et al biorXiv 2021.03.02.433398

   Inserts

   S. pyogenes sgRNA cassette
   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.23-GW

   Plasmid

   #60323

   Purpose

   This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHCD1B

   Plasmid

   #102953

   Purpose

   mammalian expression of CD1B

   Depositor

   Lucia Mori, Gennaro De Libero

   Article

   Manolova et al Eur J Immunol. 2003 Jan;33(1):29-37.

   Insert

   CD1B cDNA (CD1B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

   Plasmid

   #60910

   Purpose

   dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   dSV40 Promoter

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJExpress414_GmPOPB

   Plasmid

   #92234

   Purpose

   Express wild type prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata
   

   Use

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid

    #230026

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGW-TurboID-GOI

    Plasmid

    #209380

    Purpose

    Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV0328

    Plasmid

    #133489

    Purpose

    Expression of nucleo-cytoplasmic mCherry

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMPRA1-CMV-Clover

    Plasmid

    #53538

    Purpose

    Fluorescent reporter control for pMPRA1

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    Clover GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. SauCas9 EMX1-pegRNA

    Plasmid

    #169857

    Purpose

    SauCas9 pegRNA for EMX1

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SauCas9 EMX1-pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-AcGFP-Sec61-IRES-Blast

    Plasmid

    #154009

    Purpose

    Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    acGFP-Sec61 (SEC61B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Reb_79 aka PAS620

    Plasmid

    #71225

    Purpose

    Reb_1 with RebA(Q91R)

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebA( G53A, Q91R)

    Promoter

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pWPT-nlsLacZ

    Plasmid

    #12261

    Depositor

    Didier Trono

    Insert

    LacZ
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMD14

    Plasmid

    #138654

    Purpose

    phiOZJ integrating Streptomyces expression vector, intact RDF

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PermE* variant sequence from pDA1652

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

    Plasmid

    #70085

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe

    Depositor

    Anne Mason, Ross MacGillivray

    Article

    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

    Insert

    mutated human serum transferrin (TF Human)
    

    Use

    Tags

    Hexa His tag and N-terminal signal peptide, 4 aa …

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…

    Promoter

    SV40

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQCi-mB2m-sgRNA

    Plasmid

    #154090

    Purpose

    pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA targeting murine B2M locus
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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