163,991 results

1. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2260

   Plasmid

   #141998

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORANGE GFP-Grin2b KI

   Plasmid

   #131487

   Purpose

   Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin2b Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pED9x (dCas9-KRAB-mCherry)

   Plasmid

   #163956

   Purpose

   Lentiviral expression plasmid of sgRNA with mCherry

   Depositor

   Junwei Shi

   Article

   Giles et al Immunity. 2022 Mar 8;55(3):557-574.e7. d

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ef1a_Large T-antigen_Ires_Puro

   Plasmid

   #18922

   Depositor

   Linzhao Cheng

   Article

   Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

   Insert

   Large T Antigen (SV40gp6 Simian virus 40)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL-MHCK7-GCaMP6

   Plasmid

   #65042

   Purpose

   Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

   Depositor

   Nenad Bursac

   Article

   Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

   Insert

   GCaMP6
   

   Use

   Lentiviral

   Tags

   6xHis, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   Promoter

   MHCK7

   Available since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV PHP.eB)

   Viral Prep

   #20298-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   EF1a

   Tags

   EYFP (Cre-dependent)

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

8. pAAV/D377Y-mPCSK9

   Plasmid

   #58376

   Purpose

   Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

   Depositor

   Jacob Bentzon

   Article

   Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

   Has Service

   AAV8

   Insert

   proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   D377Y

   Promoter

   HCRApoE/hAAT

   Available since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS U6a gRNA

   Plasmid

   #158961

   Purpose

   gRNA backbone driven by zebrafish U6a promoter

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   zf U6a promoter
   gRNA backbone
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. prhaBAD-pA-Hia5

    Plasmid

    #222303

    Purpose

    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    pA-Hia5
    

    Use

    Tags

    6His and protein A

    Expression

    Bacterial

    Mutation

    codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE Tubb3-GFP KI

    Plasmid

    #131497

    Purpose

    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Tubb3, Tubb3 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

    Plasmid

    #60904

    Purpose

    The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209665

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95A.A119L

    Promoter

    hSyn

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. LCV2 cGAS KO

    Plasmid

    #217444

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human cGAS

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    sgRNA targeting cGAS (CGAS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-NUP50-N-10

    Plasmid

    #55111

    Purpose

    Localization: Nuclear Pore, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    NUP50 (NUP50 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-NTOM20-mVenus-Zdk1

    Plasmid

    #81010

    Purpose

    Expression of Zdk1 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Use

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. OmpA3-PNGaseF-TEV-His6

    Plasmid

    #114274

    Purpose

    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

    Depositor

    Martin Caffrey

    Insert

    Peptide: N-glycosidase F
    

    Use

    Tags

    OmpA3 leader sequence (for periplasmic localisati…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-NLS

    Plasmid

    #39319

    Depositor

    Martin Offterdinger

    Article

    Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

    Insert

    mCherry-3xNLS
    

    Use

    Tags

    3xSV40LT NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. ER-HA-mNG21-10

    Plasmid

    #141165

    Purpose

    Split Fluorescent Reporter for ER Retrograde Trafficking

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    ER-HA-mNG21-10-KDEL
    

    Use

    Lentiviral

    Tags

    Signal Sequence, KDEL and HA

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    Ef1a

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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