165,671 results

1. TFORF2679

   Plasmid

   #144143

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PEG3 (PEG3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2534

   Plasmid

   #142955

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOX6 (SOX6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_CMV:Flag-GeNL_SV40:PuroR

   Plasmid

   #183040

   Purpose

   Expresses the FLAG-tagged GeNL (Green Nanolantern) in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

   Insert

   GeNL Nanolantern
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xFlag-eGFP-Flag-SETX

   Plasmid

   #218838

   Purpose

   Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

   Depositor

   Albert La Spada

   Article

   Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

   Insert

   Senataxin (SETX Human)
   

   Use

   Tags

   Flag and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-MBP-TEVpCS-ADGRG6 CTFdeltaTA-FLAG

   Plasmid

   #217737

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGR6 (ADGRG6 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-846

   Promoter

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pN3-mTagBFP-pHluorin-CAAX

   Plasmid

   #178345

   Purpose

   Express a pH insensitive blue fluorencent protein mTagBFP fused to the N-terminal of a membrane targeted pH sensitive green fluorescent protein pHluorin-CAAX under CMV promoter

   Depositor

   Yulong Li

   Article

   Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

   Insert

   mTagBFP-pHluorin-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HA-MBP-TEVpCS-ADGRL1 CTFdeltaTA-FLAG

   Plasmid

   #217739

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRL1 (ADGRL1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-844

   Promoter

   Available Since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Thy1-Brainbow-1.1 M

   Plasmid

   #18726

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. LifeAct-LSSmGFP

   Plasmid

   #186534

   Purpose

   Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Use

   Tags

   LifeAct

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA-MBP-TEVpCS-ADGRL2 CTFdeltaTA-FLAG

    Plasmid

    #217740

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL2 (ADGRL2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-830

    Promoter

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. UBC plasmid

    Plasmid

    #169746

    Purpose

    Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-IRES-GFP/CALR del52

    Plasmid

    #214700

    Purpose

    Mammalian expression of human CALR del52

    Depositor

    Norio Komatsu

    Article

    Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

    Insert

    CALR (CALR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-IRES-GFP/CALR wt

    Plasmid

    #214699

    Purpose

    Mammalian expression of human CALR wt

    Depositor

    Norio Komatsu

    Article

    Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

    Insert

    CALR wt (CALR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EGFP-Talin head-SpyCatcher003

    Plasmid

    #133566

    Purpose

    Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    EGFP-Talin head-SpyCatcher003
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. OA984-HA

    Plasmid

    #120362

    Purpose

    Expresses a his-tagged single chain antibody 1C19 that targets all four DENV serotypes and expresses red fluorescent protein reporter

    Depositor

    Omar Akbari

    Article

    Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

    Insert

    1C19 his-tagged single chain antibody
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    AAEL010782 carboxypeptidase A (CPA) gene Promoter

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBbS5a-Opto-Cre-Vvd-2

    Plasmid

    #160400

    Purpose

    Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

    Depositor

    Mary Dunlop

    Article

    Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

    Insert

    Opto-Cre-Vvd-2
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET_mNG2(1-10)_32aalinker_mNG2(11)

    Plasmid

    #82611

    Purpose

    Bacterial expression vector for split mNeonGreen2 screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    split before mutations

    Promoter

    T7

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFnCpf1GG

    Plasmid

    #131010

    Purpose

    Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of FnCas12a
    GFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. C1ORF43-GFP

    Plasmid

    #130879

    Purpose

    Expresses human C1ORF43 with C-terminal GFP tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    C1ORF43 (C1orf43 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ACER

    Pooled Library

    #226117

    Purpose

    The Advanced Catalogue of Epigenetic Regulators (ACER) library allows scientists to study how different epigenetic proteins influence gene activity and contribute to diseases.

    Depositor

    Rui Lu

    Article

    Zhou et al Blood. 2024 Aug 16:blood.2023023644. doi

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only