195,273 results

1. LentiGuide-Hygro

   Plasmid

   #139462

   Purpose

   Lentiviral vector with gRNA scaffold and hygromycin selectable marker

   Depositor

   Caroline Goujon

   Insert

   no sgRNA inserted; resistance gene: hygroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCMV_LCMV_GPC

   Plasmid

   #207288

   Purpose

   Mammalian expression of Lymphocytic choriomeningitis virus GPC protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   LCMV_GPC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Roxed-Cre

   Plasmid

   #51273

   Purpose

   Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   Roxed-Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-Roxed-Cre

   Plasmid

   #51273

   Purpose

   Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   Roxed-Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E ubb polyA

   Plasmid

   #195977

   Purpose

   Gateway p3E vector

   Depositor

   Christian Mosimann, Alexa Burger

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   ubb:polyA (ubb Zebrafish)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. (189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)

   Plasmid

   #160739

   Purpose

   Expresses a truncated OGT (4.5 TPRs) fused to a GFP nanobody

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   HA-nGFP-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIRCgp41-1

   Plasmid

   #74227

   Purpose

   Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   gp41-1 C-intein and gp41-1 N-intein

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Non-specific sgRNA

   Plasmid

   #109432

   Purpose

   MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Non-specific gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-26b-NbVHH05-ALFA-His

   Plasmid

   #171569

   Purpose

   Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-ALFA-His (UBE2J1 Human)
   

   Use

   Tags

   ALFA-tag/His-tag and PelB signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. (344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

    Plasmid

    #168097

    Purpose

    HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nGFP-(EAAAK)4-OGA(544-706)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV5 HA FoxO1

    Plasmid

    #14936

    Depositor

    Joan Massague

    Article

    Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

    Insert

    FoxO1 (Foxo1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pCMV5 HA FoxO1

    Plasmid

    #14936

    Depositor

    Joan Massague

    Article

    Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

    Insert

    FoxO1 (Foxo1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

    Plasmid

    #189630

    Purpose

    Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV, Cre/Lox, and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and hSyn

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. NET47 pEGFP-N2 (593)

    Plasmid

    #61986

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET47 (TM7SF2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

15. plenti-CAG-IKZF3-FLAG-IRES-GFP

    Plasmid

    #107388

    Purpose

    Lentiviral expression of IKZF3-FLAG

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF3 (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST53-LRRK2-Y1699C

    Plasmid

    #25048

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Tyrosine 1699 mutated to Cysteine

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pDEST53-LRRK2-Y1699C

    Plasmid

    #25048

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Tyrosine 1699 mutated to Cysteine

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pCas3cRh

    Plasmid

    #133773

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Joseph Bondy-Denomy

    Article

    Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    Cas7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

    Plasmid

    #55209

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xAsn, 10xHis, MPB, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

    Plasmid

    #55209

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xAsn, 10xHis, MPB, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge