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pET263-pUC57 24xMS2V7
(Plasmid #140705)


Item Catalog # Description Quantity Price (USD)
Plasmid 140705 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2749
  • Total vector size (bp) 4177
  • Modifications to backbone
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    24 MS2 loops
  • Species
  • Insert Size (bp)
  • Mutation
    24 MS2 loops V7, high-affinity C-variant, inter-spaced by 41 nucleotide linkers, no stop codons in the 3 reading frames.
  • Promoter None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
  • 3′ sequencing primer CAGGAAACAGCTATGACCATG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET263-pUC57 24xMS2V7 was a gift from Robert Singer & Evelina Tutucci (Addgene plasmid # 140705 ; ; RRID:Addgene_140705)
  • For your References section:

    An improved MS2 system for accurate reporting of the mRNA life cycle. Tutucci E, Vera M, Biswas J, Garcia J, Parker R, Singer RH. Nat Methods. 2017 Nov 13. doi: 10.1038/nmeth.4502. 10.1038/nmeth.4502 PubMed 29131164