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(Plasmid #84561)


Item Catalog # Description Quantity Price (USD)
Plasmid 84561 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pUbC lentivirus expression vector (2nd generation)
  • Backbone size w/o insert (bp) 6255
  • Total vector size (bp) 11219
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy


  • Gene/Insert name
    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
  • Alt name
  • Species
    M. musculus (mouse), A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
  • Promoter UbC
  • Tag / Fusion Protein
    • Flag, Suntag, oxBFP, AID (auxin-inducible degron)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ClaI--but may not be unique due to multiple inserts (not destroyed)
  • 5′ sequencing primer cgc cga tga tta tat aag ga (UbC)
  • 3′ sequencing primer cat aaa gag aca gca acc ag (Wpre)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre was a gift from Robert Singer (Addgene plasmid # 84561 ; ; RRID:Addgene_84561)
  • For your References section:

    Translation dynamics of single mRNAs in live cells and neurons. Wu B, Eliscovich C, Yoon YJ, Singer RH. Science. 2016 Jun 17;352(6292):1430-5. doi: 10.1126/science.aaf1084. Epub 2016 May 5. 10.1126/science.aaf1084 PubMed 27313041