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Translation dynamics of single mRNAs in live cells and neurons.
Wu B, Eliscovich C, Yoon YJ, Singer RH
Science. 2016 Jun 17;352(6292):1430-5. doi: 10.1126/science.aaf1084. Epub 2016 May 5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
84561pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-WprepSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops
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84563pUbC-OsTIR1-myc-IRES-scFv-sfGFPBicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP
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Antibodies from Article