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phage-ubc-nls-ha-tdMCP-gfp
(Plasmid #40649)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40649 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHAGE-UBC-RIG (modified)
  • Modifications to backbone
    see associated publication
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tdMCP-gfp
  • Alt name
    tandem dimer MS2 coat protein (MCP)
  • Species
    Synthetic
  • Promoter human ubiquitin C (UBC)
  • Tags / Fusion Proteins
    • NLS (N terminal on backbone)
    • HA (N terminal on backbone)
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer FUGW (5'-ATTACAGGGACAGCAGAGATCC-3')
  • 3′ sequencing primer WPRE-R (5'CATAGCGTAAAAGGAGCAACA-3')
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The linker region between the two MCPs is ATCTACGCCATGGCTTCT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phage-ubc-nls-ha-tdMCP-gfp was a gift from Robert Singer (Addgene plasmid # 40649 ; http://n2t.net/addgene:40649 ; RRID:Addgene_40649)
  • For your References section:

    Fluorescence fluctuation spectroscopy enables quantitative imaging of single mRNAs in living cells. Wu B, Chao JA, Singer RH. Biophys J. 2012 Jun 20;102(12):2936-44. Epub 2012 Jun 19. 10.1016/j.bpj.2012.05.017 PubMed 22735544