pUbC-nls-ha-stdMCP-stdGFP
(Plasmid #98916)

• Purpose: synonymized tandem dimer MCP fused to synonymized tandem dimer GFP
• Depositing Lab: Robert Singer
• Publication: Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi: 10.1101/gad.259358.115. Epub 2015 Apr 15.

Backbone

• Vector backbone: pUbC
• Backbone size w/o insert (bp): 6250
• Total vector size (bp): 8536
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: stdMCP-stdGFP
• Species: Synthetic
• Insert Size (bp): 2283
• Promoter: UbC
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: ClaI (unknown if destroyed)
• 5′ sequencing primer: cgc cga tga tta tat aag ga (UbC)
• 3′ sequencing primer: cat aaa gag aca gca acc ag (Wpre)

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry