Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
(Plasmid #104394)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104394 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    YcpLac111
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCP-NLS-2xyeGFP
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Insert Size (bp)
    1827
  • Mutation
    MCP fused to NLS(SV40) and 2x yeast codon optimized eGFP
  • Promoter CYC1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuII (unknown if destroyed)
  • 3′ cloning site PvuII (not destroyed)

Resource Information

Depositor Comments

Insert expressed under the control of the CYC1 promoter and terminator

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP was a gift from Robert Singer (Addgene plasmid # 104394 ; http://n2t.net/addgene:104394 ; RRID:Addgene_104394)
  • For your References section:

    An improved MS2 system for accurate reporting of the mRNA life cycle. Tutucci E, Vera M, Biswas J, Garcia J, Parker R, Singer RH. Nat Methods. 2017 Nov 13. doi: 10.1038/nmeth.4502. 10.1038/nmeth.4502 PubMed 29131164