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Addgene

pcDNA-TRBP
(Plasmid #15666)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15666 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1 (+)
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRBP
  • Alt name
    TARBP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1101
  • Entrez Gene
    TARBP2 (a.k.a. LOQS, TRBP, TRBP1, TRBP2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-TRBP was a gift from Dong-Yan Jin (Addgene plasmid # 15666 ; http://n2t.net/addgene:15666 ; RRID:Addgene_15666)
  • For your References section:

    Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA. Kok KH, Ng MH, Ching YP, Jin DY. J Biol Chem. 2007 Jun 15. 282(24):17649-57. 10.1074/jbc.M611768200 PubMed 17452327