Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15667||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 (+)
- Backbone size w/o insert (bp) 5428
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)942
Entrez GenePRKRA (a.k.a. DYT16, HSD14, PACT, RAX)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-PACT was a gift from Dong-Yan Jin (Addgene plasmid # 15667 ; http://n2t.net/addgene:15667 ; RRID:Addgene_15667)
For your References section:Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA. Kok KH, Ng MH, Ching YP, Jin DY. J Biol Chem. 2007 Jun 15. 282(24):17649-57. 10.1074/jbc.M611768200 PubMed 17452327