168,567 results

1. pLL3.7 EGFPC2 TAZ

   Plasmid

   #66850

   Purpose

   Express GFP-fused TAZ by lentivirus

   Depositor

   Yutaka Hata

   Article

   Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

   Insert

   WWTR1/TAZ (WWTR1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV/eGFP-U6-sgRNA

   Plasmid

   #170544

   Purpose

   A lentiviral backbone for homology directed insertion of eGFP. Containing only eGFP, flanked by restriction sites for insertion of homology arms and a sgRNA casstte to clone in sgRNA for HDR

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCitrine-pBAD

   Plasmid

   #54723

   Purpose

   Localization: Protein Expression Vector , Excitation: 516, Emission: 529

   Depositor

   Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

   Article

   Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

   Type

   Empty backbone

   Tags

   6xHis and mCitrine

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLVX-XBP1 mNeonGreen NLS

   Plasmid

   #115968

   Purpose

   ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   X-box binding protein 1 (XBP1 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   HA tag, c-myc NLS, and mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-BRD2

   Plasmid

   #65376

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD2 (BRD2 Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHCMV-VSVgSS-15F11-TM

   Plasmid

   #207317

   Purpose

   Functional incorporation of 15F11 scFv (anti-HA) into envelope of virions

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   15F11 ScFv
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF3138

   Plasmid

   #144614

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PPARG (PPARG Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. M23: pHR-SFFVp-FUSN::mCherry::SspB

   Plasmid

   #122148

   Purpose

   N terminal domain of FUS (1-214) tagged with SspB and mCherry.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FUS (FUS Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 1-214

   Promoter

   SFFV

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 2Flag-pDEST-C

   Plasmid

   #118372

   Purpose

   Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   2Flag

   Expression

   Mammalian

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-NBRE3

    Plasmid

    #208254

    Purpose

    pGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cells

    Depositor

    Douglas Kojetin

    Article

    Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

    Insert

    3X NBRE-LUC
    

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Plenti-CMV-MCS-RFP-SV-puro

    Plasmid

    #109377

    Purpose

    This plasmid expresses dsRed2 and puromycin resistance

    Depositor

    Jonathan Garlick, Behzad Gerami-Naini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    dsRed2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tet1-dCas9

    Plasmid

    #136650

    Purpose

    Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Tet1-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.hSynapsin1.OxLight1

    Plasmid

    #169792

    Purpose

    Fluorescent reporter for orexin neuropeptides

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

    Insert

    OxLight1
    

    Use

    AAV

    Promoter

    human Synapsin-1

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-hChR2(H134R)-mCherry (AAV9)

    Viral Prep

    #26976-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-hChR2(H134R)-mCherry (#26976). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-mCherry plasmid DNA. Synapsin-driven, humanized channelrhodopsin H134R mutant, fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Syn

    Tags

    mCherry

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pLHCX-XBP1 mNeonGreen NLS

    Plasmid

    #115971

    Purpose

    ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    X-box binding protein 1 (XBP1 Synthetic, Human)
    

    Use

    Retroviral

    Tags

    HA tag, c-myc NLS, and mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-28a(+)-FGF2-K128N

    Plasmid

    #120284

    Purpose

    Produce human recombinant FGF2 K128N in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Basic fibroblast growth factor 2 (FGF2 Synthetic, Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1a mCherry

    Plasmid

    #129431

    Purpose

    expression of mCherry from EF1a promoter

    Depositor

    Oskar Laur

    Insert

    mCherry
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQFT-mNG

    Plasmid

    #203675

    Purpose

    pQFT with mNeonGreen under control of PQJ

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Insert

    mNeonGreen
    

    Expression

    Bacterial

    Promoter

    PQJ

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENN.AAV.CMVs.Pl.Cre.rBG (AAV2)

    Viral Prep

    #105537-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

20. GPR132-Tango

    Plasmid

    #66315

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR132 (GPR132 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only