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Plasmid#155342PurposePlasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.DepositorInserthumanized EBpaRS/BstYam
UseTagsExpressionMammalianMutationPromoterPGK/U6Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EGFP-Bulged-miR-19
Plasmid#91976PurposeEGFP reporter cell line with eight bulged miR-19 sitesDepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap-FLEX.SF-iGluSnFR.A184S
Plasmid#106180PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV5InsertSF-iGluSnFR.A184S
UseAAVTagsExpressionMutationGltI: A184SPromoterhSynapsinAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP
Plasmid#85482PurposeArabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterArabinoseAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
EFSp-GFP-YAP (5SA)
Plasmid#174170PurposeLentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationFive LATS phosphorylation sites (S61, S109, S127,ā¦PromoterEFSAvailable sinceSept. 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
p5E-CMVSP6
Plasmid#138356PurposeGateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)DepositorInsertCMV-SP6 promoter
UseGateway compatibleTagsExpressionMutationPromoterCMV-SP6Available sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-10A1
Plasmid#15805DepositorInsert10A1 Murine leukemia virus envelope mRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)
Plasmid#181745Purposedual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1DepositorInserthuman/zebrafish codon optimized SpCas9
UseIn vitro transcription; t7 promoterTagsNLS(SV40)-3xFLAGExpressionBacterialMutationPromoterT7Available sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD555 ZF150x6-Compact_EYFP
Plasmid#138869PurposeEYFP reporter vector for the ZF150x6-Compact promoterDepositorInsertZF150x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF150x6-CompactAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX333-T2A-EGFP
Plasmid#197417PurposeSpCas9-T2A-EGFP with dual sgRNA cloning backbonesDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCBh; U6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMLH1
Plasmid#129426PurposeExpression human MLH1 protein using baculovirusDepositorInserthuman MLH1 (MLH1 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGAMS-mEos2
Plasmid#105128PurposeExpresses human FGAMS-mEos2 fusion in mammalian cellsDepositorInsertPFAS (PFAS Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SOX2
Plasmid#26817DepositorInsert5'UTR-SOX2-3'UTR (SOX2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO_FOXA1_#2
Plasmid#70096PurposeExpression of shRNA to human FOXA1, puromycin selectionDepositorInsertFOXA1 (FOXA1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoteru6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
hSyn-BiPOLES-mCerulean
Plasmid#154944PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine
Plasmid#50472PurposeGs-coupled rM3D-IRES-mCitrine under the control of GFAP promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-Cas9Bsd-W
Plasmid#68343PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termiā¦ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-dTom-nlsdTom (AAV PHP.eB)
Viral Prep#135630-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Lifeact-EGFP-2XNLS
Plasmid#58467PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoterDepositorInsertsLifeact
2X NLS with 2X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPD541 ZF13x6-Compact_EYFP
Plasmid#138855PurposeEYFP reporter vector for the ZF13x6-Compact promoterDepositorInsertZF13x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF13x6-CompactAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only