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Plasmid#180375PurposePseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike proteinDepositorInsertSpike (omicron BA.1 variant) (S SARS-CoV-2)
UseLentiviral and RetroviralTagsExpressionMammalianMutationc terminal D18PromoterCMVAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-N346A/C348A
Plasmid#127411PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethersDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationN346A-C348APromoteraraBADAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-GFP-Clathrin
Plasmid#170858PurposeMammalian expression of His-GFP-Clathrin.DepositorInsertClathrin light polypeptide (Lca) (Clta Mouse)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV5)
Viral Prep#35509-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
Plasmid#60910PurposedCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag systemDepositorInsertdCas9
UseCRISPR and LentiviralTags2x NLS, HA, and NLSExpressionMammalianMutationPromoterdSV40 PromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
Laconic/pcDNA3.1(-)
Plasmid#44238Purposea genetically-encoded sensor for lactate concentration, transport and metabolic fluxDepositorInsertLaconic (LACtate Optical Nano Indicator from CECs)
UseLactate biosensorTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GAPDH
Plasmid#83910Purposestable overexpressionDepositorInsertglyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
p406ADH1
Plasmid#15974DepositorInsertADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationADH1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.LSL.EGFP
Plasmid#100047PurposeAAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)DepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-MitoTimer
Plasmid#50547PurposeExpresses the Timer protein targeted to the mitochondria under a Tet-inducible promoterDepositorInsertMitoTimer
UseTagsExpressionMammalianMutationF12S mutation in TimerPromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
hCathepsin B
Plasmid#11249PurposeMammalian expression of human cathepsin BDepositorInsertcathepsin B (CTSB Human)
UseTagsC9 (GTETSQVAPA)ExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-EF1a-eFFly-mCherry
Plasmid#104833Purpose3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detectionDepositorInsertseFFly
mCherry
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
HyGCaMP6s
Plasmid#102558PurposeExpression of GCaMP6s in HydraDepositorInsertGCaMP6s
UseHydra expressionTagsActin/GCaMP6s fusion protein (C terminal on backb…ExpressionMutationPromoterAvailable sinceOct. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CTCF
Plasmid#116728PurposeLentiviral expression of CTCFDepositorInsertCTCF (CTCF Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-Jaws-KGC-GFP-ER2 (AAV Retrograde)
Viral Prep#65014-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hsyn-Jaws-KGC-GFP-ER2 (#65014). In addition to the viral particles, you will also receive purified pAAV-hsyn-Jaws-KGC-GFP-ER2 plasmid DNA. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFPAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVSV-G
Plasmid#138479PurposeExpresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.DepositorInsertVSV-G envelop protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFlag-eGFP-Flag-SETX
Plasmid#218838PurposeMammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.DepositorInsertSenataxin (SETX Human)
UseTagsFlag and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR
Plasmid#213480PurposeAAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.DepositorInsertsmtagBFP-KASH
U6-gRNA-directcapture
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMetYC-Dest
Plasmid#105081PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.DepositorTypeEmpty backboneUseTagsCub-PLVExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJRD215
Plasmid#167555PurposeBackbone plasmid to pRMA154DepositorTypeEmpty backboneUseBroad host range mobilisableTagsExpressionMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only