166,367 results

1. Lenti:FlipGFP-T2A-mCherry

   Plasmid

   #223198

   Purpose

   Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

   Depositor

   Yeh-Hsing Lao

   Article

   Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

   Insert

   FlipGFP-T2A-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. H2B-GFP

   Plasmid

   #11680

   Depositor

   Geoff Wahl

   Article

   Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

   Insert

   Human histone H2B (H2BC11 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.GFAP.Cre.WPRE.hGH (AAV PHP.eB)

   Viral Prep

   #105550-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV.GFAP.Cre.WPRE.hGH (#105550). In addition to the viral particles, you will also receive purified pAAV.GFAP.Cre.WPRE.hGH plasmid DNA. GFAP-driven expression of Cre. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   GFAP

   Tags

   None

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

4. pPB-cT3G-cERP2-RUNX2

   Plasmid

   #192926

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   RUNX2 (RUNX2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-SARS-CoV-2-S (Wuhan)

   Plasmid

   #215812

   Purpose

   Expresses SARS-CoV-2 Spike protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-SYN-sDarken

   Plasmid

   #184796

   Purpose

   Serotonin Sensor sDarken under the control of a hSyn Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   sDarken
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCoPURO

   Plasmid

   #17533

   Depositor

   Francis Castellino

   Article

   Iwaki et al Biotechniques. 2003 Sep . 35(3):482-4, 4

   Insert

   Puromycine Acetyl Transferase
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBZS-YP_009702000

   Plasmid

   #198379

   Purpose

   Express YP_009702000 in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   YP_009702000 (CVA-1_913R )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor

   Plasmid

   #202689

   Purpose

   CLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.

   Depositor

   Stanley Qi

   Article

   Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

   Inserts

   CopGFP
   mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ8079 - IL2RG GFP-P2A CLIP Donor

    Plasmid

    #202690

    Purpose

    CLIP donor template for integrating GFP into IL2RG locus.

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Insert

    CopGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORTMAGE-3

    Plasmid

    #72678

    Purpose

    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Promoter

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pInt-ts

    Plasmid

    #66076

    Purpose

    The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

    Insert

    lambda integrase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLIB-HIS-TEV-scCAK1

    Plasmid

    #177014

    Purpose

    Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
    

    Use

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCHW91013

    Plasmid

    #222450

    Purpose

    Level 1, Position 3 containing CBS4-miniDFR::RUBY::OCS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    CBS4-miniDFR::RUBY::OCS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB213

    Plasmid

    #101677

    Purpose

    mCherry under T7 promoter control

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    mCherry under T7 promoter control
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIVTRup

    Plasmid

    #101362

    Purpose

    Highly efficient in vitro transcription. Empty backbone.

    Depositor

    Ángel Raya

    Insert

    5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
    

    Use

    For cloning a gene for in vitro transcription pur…

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-ERK1

    Plasmid

    #14747

    Depositor

    Rony Seger

    Article

    Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

    Insert

    ERK1 (MAPK3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51083

    Purpose

    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. KW57_HART_RAC1

    Plasmid

    #207399

    Purpose

    Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR

    Depositor

    Stephen Floor

    Article

    Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-dCas9

    Plasmid

    #47106

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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