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L1-neo-TET
(Plasmid #51284)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51284 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    L1RE1
  • Alt name
    L1
  • Alt name
    Line1
  • Species
    H. sapiens (human)
  • Entrez Gene
    L1RE1 (a.k.a. L1.2, LRE1)
  • Promoter CMV
  • Tag / Fusion Protein
    • neoTET cassette with a tetrahymena self-splicing intron interrupting the neo gene (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

L1 is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L1-neo-TET was a gift from Astrid Roy-Engel (Addgene plasmid # 51284 ; http://n2t.net/addgene:51284 ; RRID:Addgene_51284)
  • For your References section:

    The RNA polymerase dictates ORF1 requirement and timing of LINE and SINE retrotransposition. Kroutter EN, Belancio VP, Wagstaff BJ, Roy-Engel AM. PLoS Genet. 2009 Apr;5(4):e1000458. doi: 10.1371/journal.pgen.1000458. Epub 2009 Apr 24. 10.1371/journal.pgen.1000458 PubMed 19390602