Purposeexpresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51283||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesH. sapiens (human)
- Promoter upstream pol III enhancer sequence of the 7SL gene (113 bp)
/ Fusion Protein
- neoTET cassette with a tetrahymena self-splicing intron interrupting the neo gene (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer M13-pUC-Fwd
- 3′ sequencing primer SV40pro-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Alu is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs.
Please note that the cassette is in the reverse orientation within the MCS relative to the full sequence--This has no functional consequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAluYa5-neo-TET was a gift from Astrid Roy-Engel (Addgene plasmid # 51283 ; http://n2t.net/addgene:51283 ; RRID:Addgene_51283)
For your References section:The RNA polymerase dictates ORF1 requirement and timing of LINE and SINE retrotransposition. Kroutter EN, Belancio VP, Wagstaff BJ, Roy-Engel AM. PLoS Genet. 2009 Apr;5(4):e1000458. doi: 10.1371/journal.pgen.1000458. Epub 2009 Apr 24. 10.1371/journal.pgen.1000458 PubMed 19390602