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The RNA polymerase dictates ORF1 requirement and timing of LINE and SINE retrotransposition.
Kroutter EN, Belancio VP, Wagstaff BJ, Roy-Engel AM
PLoS Genet. 2009 Apr;5(4):e1000458. doi: 10.1371/journal.pgen.1000458. Epub 2009 Apr 24.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51283pAluYa5-neo-TETexpresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
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51284L1-neo-TETexpresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette
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51285p7SL-neo-TETexpresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette
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51286pU6-neo-TETexpresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette
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51287pB2-neo-TETexpresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
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Antibodies from Article