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pBudORF2-CH
(Plasmid #51289)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51289 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBudCE4.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4595
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin(Zeocin)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    L1 ORF2
  • Alt name
    L1RE1
  • Alt name
    Line1
  • Species
    H. sapiens (human)
  • Entrez Gene
    L1RE1 (a.k.a. L1.2, LRE1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Myc (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

This plasmid was created using the codon optimized L1RP as a source for the ORF2 coding sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBudORF2-CH was a gift from Astrid Roy-Engel (Addgene plasmid # 51289 ; http://n2t.net/addgene:51289 ; RRID:Addgene_51289)
  • For your References section:

    Evolutionary conservation of the functional modularity of primate and murine LINE-1 elements. Wagstaff BJ, Barnerssoi M, Roy-Engel AM. PLoS One. 2011 May 10;6(5):e19672. doi: 10.1371/journal.pone.0019672. 10.1371/journal.pone.0019672 PubMed 21572950