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(Plasmid #51290)


Item Catalog # Description Quantity Price (USD)
Plasmid 51290 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4595
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    L1 ORF1
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Entrez Gene
    L1RE1 (a.k.a. L1.2, LRE1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Although a myc tag is present in the full sequence, this tag is not present in this plasmid.

pBudORF1-CH was generated by cloning the polymerase chain reaction (PCR)-amplified codon-optimized consensus sequences of each ORF into the HindIII-BamHI sites of the pBudCE4.1 vector in a manner that removes the stop codon of the ORF1, so the expressed protein will contain the his tag at the carboxy terminus. The following primers were used in the amplification of ORF1: 5-AGACCCAAGCTTAGCTAAAACCACAAAGATG-3′ and 5-TGTTCGGATCCGATCTTGGTGTGCTTCTGCAGGGG-3′

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBudORF1-CH was a gift from Astrid Roy-Engel (Addgene plasmid # 51290 ; ; RRID:Addgene_51290)
  • For your References section:

    Molecular reconstruction of extinct LINE-1 elements and their interaction with nonautonomous elements. Wagstaff BJ, Kroutter EN, Derbes RS, Belancio VP, Roy-Engel AM. Mol Biol Evol. 2013 Jan;30(1):88-99. doi: 10.1093/molbev/mss202. Epub 2012 Aug 23. 10.1093/molbev/mss202 PubMed 22918960