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BPK3082
(Plasmid #78742)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78742 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MLM3636
  • Backbone manufacturer
    Joung lab (Addgene Plasmid # 43860)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LbCpf1 crRNA backbone, without spacer sequence
  • Species
    Synthetic
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (not destroyed)
  • 3′ cloning site BsmBI (not destroyed)
  • 5′ sequencing primer OS280 (5'-CAGGGTTATTGTCTCATGAGCGG-3')
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Use BsmBI sites to insert crRNA spacer sequence into the vector. This plasmid will express gRNA in mammalian cells for Cpf1 from Lachnospiraceae bacterium ND2006. Recommended to use with SQT1665 (Addgene plasmid# 78744), which expresses Lachnospiraceae bacterium ND2006 Cpf1.

Please visit https://www.biorxiv.org/content/early/2016/06/09/057802 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BPK3082 was a gift from Keith Joung (Addgene plasmid # 78742 ; http://n2t.net/addgene:78742 ; RRID:Addgene_78742)
  • For your References section:

    Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells. Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK. Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620. 10.1038/nbt.3620 PubMed 27347757