PurposeHuman expression plasmid for AsCpf1-NLS-3xHA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78743||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerCepko lab (Addgene plasmid# 11179)
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemammalian codon-optimized Acidaminococcus sp. BV3L6 Cpf1-NLS-3xHA
SpeciesAcidaminococcus sp. BV3L6
- Promoter CAG
/ Fusion Proteins
- NLS (C terminal on insert)
- 3x HA (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
This plasmid expresses Cpf1 from Acidaminococcus sp. BV3L6 in mammalian cells. Recommended to use with BPK3079 (Addgene plasmid# 78741), which can be used to express a gRNA for AsCpf1.
Please visit https://www.biorxiv.org/content/early/2016/06/09/057802 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SQT1659 was a gift from Keith Joung (Addgene plasmid # 78743 ; http://n2t.net/addgene:78743 ; RRID:Addgene_78743)
For your References section:Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells. Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK. Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620. 10.1038/nbt.3620 PubMed 27347757