BPK3079
(Plasmid #78741)

• Purpose: Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)
• Depositing Lab: Keith Joung
• Publication: Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	78741	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: MLM3636
  (Search Vector Database)
• Backbone manufacturer: Joung lab (Addgene Plasmid # 43860)
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AsCpf1 crRNA backbone, without spacer sequence
• Species: Synthetic
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (not destroyed)
• 3′ cloning site: BsmBI (not destroyed)
• 5′ sequencing primer: OS280 (5'-CAGGGTTATTGTCTCATGAGCGG-3')

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
  • Joung Lab gRNA Cloning Protocol
• Terms and Licenses:
  • UBMTA
• Articles Citing this Plasmid:
  • 2 References

Depositor Comments

Use BsmBI sites to insert crRNA spacer sequence into the vector. This plasmid will express gRNA in mammalian cells for Cpf1 from Acidaminococcus sp. (strain BV3L6). Recommended to use with SQT1659 (Addgene plasmid# 78743), which expresses Acidaminococcus sp. Cpf1.

Please visit https://www.biorxiv.org/content/early/2016/06/09/057802 for bioRxiv preprint.