PurposeHuman expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78741||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerJoung lab (Addgene Plasmid # 43860)
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameAsCpf1 crRNA backbone, without spacer sequence
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (not destroyed)
- 3′ cloning site BsmBI (not destroyed)
- 5′ sequencing primer OS280 (5'-CAGGGTTATTGTCTCATGAGCGG-3') (Common Sequencing Primers)
Use BsmBI sites to insert crRNA spacer sequence into the vector. This plasmid will express gRNA in mammalian cells for Cpf1 from Acidaminococcus sp. (strain BV3L6). Recommended to use with SQT1659 (Addgene plasmid# 78743), which expresses Acidaminococcus sp. Cpf1.
Please visit https://www.biorxiv.org/content/early/2016/06/09/057802 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BPK3079 was a gift from Keith Joung (Addgene plasmid # 78741 ; http://n2t.net/addgene:78741 ; RRID:Addgene_78741)
For your References section:Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells. Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK. Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620. 10.1038/nbt.3620 PubMed 27347757