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  • Tatjana Sauka-Spengler Lab Plasmids

Tatjana Sauka-Spengler Lab Plasmids

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Title Authors Publication
Biotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, Senanayake U, Fraser SE, Sauka-Spengler T Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045.
Sauka-Spengler lab: Generation of a double binary transgenic zebrafish model to study myeloid gene regulation in response to melanocyte transformation (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
79878pMTB2-Avi-Cerulean-RanGAPCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (Gallus gallus)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences pMT RANGAP1 Add to Cart
79880pMTB2-Avi-Cerulean-Rpl10aCerulean protein fused to zebrafish Rpl10a ribosomal unit (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences pMT rpl10a wu:fb94f08, zgc:73082, zgc:86881 Add to Cart
79881pMT-ubb-Avi-Cerulean-RanGAPCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (Gallus gallus)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences pMT RANGAP1 Add to Cart
79882pMT-ubb-Avi-Cerulean-Rpl10aCerulean protein fused to zebrafish Rpl10a ribosomal unit (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences pMT rpl10a wu:fb94f08, zgc:73082, zgc:86881 Add to Cart
79885pMT-ubb-cytoBirA-2a-mCherryBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences pMT Add to Cart
79886pMT-ubb-NLS-BirA-2a-mCherryBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences pMT Add to Cart
79887pGEM-cytoBirA-2A-mCherry-SV40pA-FKFHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene pGEM-T Add to Cart
79889pGEM-NLS-BirA-2A-mCherry-SV40pA-FKFHA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene pGEM-T Add to Cart
79890pCR4-TOPO-pTARBAC-iTol2-AmpExtended iTol2-Amp cassette for integration of Tol2 arms into BAC clones with pTARBAC backbone (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Extended iTol2-Amp cassette for integration of Tol2 arms into BAC clones with pTARBAC backbone. Cassette contains long homologous recombination arms (5’arm-224bp and 3’arm-221bp) that flank loxP sites pCR4-TOPO Add to Cart
80056pMT-flk1-cytoBirA-2A-mCherry_RasBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80058pMT-MCS-cytoBirA-2A-mCherry_RasBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80059pMT-MCS-NLS-BirA-2A-mCherry_RasBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80060pMT-myl7-cytoBirA-2A-mCherry_RasBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80061pMT-myl7-NLS-BirA-2A-mCherry_RasBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80062pMT-sox10-cytoBirA-2A-mCherry_RasBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80063pMT-sox10-NLS-BirA-2A-mCherry_RasBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80064pMT-zic2a-cytoBirA-2A-mCherry_RasBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80065pMT-zic2a-NLS-BirA-2A-mCherry_RasBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80066pMTB2-cytoBirA-2A-mCherry_RasBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80067pMTB2-NLS-BirA-2A-mCherry_RasBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80068pMTB2-RanGAP-Cerulean-AviCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (Gallus gallus)Bacterial ExpressionBiotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences pMT RANGAP1 Add to Cart
89886pCrysβ:ECFP-LexOP:mCherry-NRasQ61KmCherry-NRasQ61K (Homo sapiens)Unspecified Sauka-Spengler lab: Generation of a double binary transgenic zebrafish model to study myeloid gene regulation in response to melanocyte transformation (unpublished) Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening. pDs(cry:ECFP-LexOP:Cherry) Add to Cart
89887pCrysβ:ECFP-LexOP:mCherry-HrasG12VmCherry-HRasG12V (Homo sapiens)Unspecified Sauka-Spengler lab: Generation of a double binary transgenic zebrafish model to study myeloid gene regulation in response to melanocyte transformation (unpublished) Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening. pDs(cry:C-LOP:Ch) Add to Cart
89888pCrysβ:ECFP-LexOP:mCherry-KrasG12VmCherry-KRasG12V (Homo sapiens)Unspecified Sauka-Spengler lab: Generation of a double binary transgenic zebrafish model to study myeloid gene regulation in response to melanocyte transformation (unpublished) Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening. pDs(cry:C-LOP:Ch) Add to Cart
89890pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRTHA-BirA-2A-citrine (Danio rerio)Unspecified Sauka-Spengler lab: Generation of a double binary transgenic zebrafish model to study myeloid gene regulation in response to melanocyte transformation (unpublished) BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette. pGEM-GFP-SV40pA-FRT-Kan-FRT Add to Cart