This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

Tatjana Sauka-Spengler Lab Plasmids


The Tatjana Sauka-Spengler Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

Learn more about research in the Tatjana Sauka-Spengler Lab.

Addgene Alerts

You must login or create an account to subscribe for Addgene Alerts.

ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
79878pMTB2-Avi-Cerulean-RanGAPCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (Gallus gallus)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences pMT RANGAP1 Add to Cart
79880pMTB2-Avi-Cerulean-Rpl10aCerulean protein fused to zebrafish Rpl10a ribosomal unit (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences pMT rpl10a wu:fb94f08, zgc:73082, zgc:86881 Add to Cart
79881pMT-ubb-Avi-Cerulean-RanGAPCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (Gallus gallus)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences pMT RANGAP1 Add to Cart
79882pMT-ubb-Avi-Cerulean-Rpl10aCerulean protein fused to zebrafish Rpl10a ribosomal unit (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences pMT rpl10a wu:fb94f08, zgc:73082, zgc:86881 Add to Cart
79885pMT-ubb-cytoBirA-2a-mCherryBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences pMT Add to Cart
79886pMT-ubb-NLS-BirA-2a-mCherryBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences pMT Add to Cart
79887pGEM-cytoBirA-2A-mCherry-SV40pA-FKFHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene pGEM-T Add to Cart
79889pGEM-NLS-BirA-2A-mCherry-SV40pA-FKFHA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene pGEM-T Add to Cart
79890pCR4-TOPO-pTARBAC-iTol2-AmpExtended iTol2-Amp cassette for integration of Tol2 arms into BAC clones with pTARBAC backbone (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Extended iTol2-Amp cassette for integration of Tol2 arms into BAC clones with pTARBAC backbone. Cassette contains long homologous recombination arms (5’arm-224bp and 3’arm-221bp) that flank loxP sites pCR4-TOPO Add to Cart
80056pMT-flk1-cytoBirA-2A-mCherry_RasBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80058pMT-MCS-cytoBirA-2A-mCherry_RasBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80059pMT-MCS-NLS-BirA-2A-mCherry_RasBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80060pMT-myl7-cytoBirA-2A-mCherry_RasBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80061pMT-myl7-NLS-BirA-2A-mCherry_RasBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80062pMT-sox10-cytoBirA-2A-mCherry_RasBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80063pMT-sox10-NLS-BirA-2A-mCherry_RasBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80064pMT-zic2a-cytoBirA-2A-mCherry_RasBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80065pMT-zic2a-NLS-BirA-2A-mCherry_RasBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80066pMTB2-cytoBirA-2A-mCherry_RasBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80067pMTB2-NLS-BirA-2A-mCherry_RasBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein (Danio rerio)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences pMT Add to Cart
80068pMTB2-RanGAP-Cerulean-AviCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (Gallus gallus)Bacterial Expression The Biotagging toolkit for analysis of specific cell populations reveals gene regulatory logic encoded in the nuclear transcriptome (unpublished) Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences pMT RANGAP1 Add to Cart