198,905 results

1. pEGFP-2xFYVE

   Plasmid

   #140047

   Purpose

   Expresses GFP-tagged HRS 2xFYVE in mammalian cells

   Depositor

   Harald Stenmark

   Article

   Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

   Insert

   tandem HRS FYVE domain (Hgs Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   two FYVE domains (amino acids 147-223), linked by…

   Promoter

   CMV

   Available since

   May 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. dTomato-2xrGBD

   Plasmid

   #129625

   Purpose

   Visualization of active RhoA

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

   Insert

   dTomato-2xrGBD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVdel

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SIRT3 Flag

   Plasmid

   #13814

   Purpose

   Mammalian expression of human SIRT3 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT3 (SIRT3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATF4 1: Mouse ATF4 (CHOP11/cATF)-WT

   Plasmid

   #21845

   Depositor

   David Ron

   Insert

   ATF4 (Atf4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHLUM

   Plasmid

   #40276

   Depositor

   Markus Ralser

   Article

   Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

   Inserts

   LEU2 (LEU2 Budding Yeast)
   URA3 (URA3 Budding Yeast)
   MET15/17 (MET17 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   endogenous LEU2, endogenous MET15/17, and endogen…

   Available since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1-MITF-M

   Plasmid

   #38131

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   MITF-M (MITF Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted stop codon

   Promoter

   CMV

   Available since

   July 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-NCad-GA

   Plasmid

   #191666

   Purpose

   ddGFP-A-inserted mouse N-cadherin

   Depositor

   Takeharu Nagai

   Article

   Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

   Insert

   N-cadherin
   

   Use

   Tags

   HA and ddGFP-A

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSynapsin1-axon-GCaMP6s (AAV1)

   Viral Prep

   #111262-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-hSynapsin1-axon-GCaMP6s (#111262). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s plasmid DNA. Synapsin-driven, axon-targeted GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Promoter

   Syn

   Tags

   None

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

9. CRISPaint Gene Tagging Kit

   Plasmid Kit

   #1000000086

   Purpose

   Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags

   Depositor

   Veit Hornung

   Available since

   Sept. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

10. lenti_dCas9-KRAB-MeCP2

    Plasmid

    #122205

    Purpose

    Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Lentiviral

    Tags

    KRAB-MeCP2

    Expression

    Mammalian

    Mutation

    Cas9 (D10A, H840A)

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. mTagBFP2-Rab5a-7

    Plasmid

    #55322

    Purpose

    Localization: Ras GTPase, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Rab5a
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. mCerulean3-C1

    Plasmid

    #54605

    Purpose

    Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Type

    Empty backbone

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. CLYBL-TO-PAX7

    Plasmid

    #204346

    Purpose

    Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    human PAX7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-NICD

    Plasmid

    #26891

    Depositor

    Nicholas Gaiano

    Article

    Dang et al Dev Neurosci. 2006;28(1-2):58-69.

    Insert

    NICD (Notch1 Mouse)
    

    Use

    Tags

    FLAG and b-globin polyA

    Expression

    Mammalian

    Mutation

    E2299K

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pLV-EF1a-H2B-miRFP670

    Plasmid

    #212668

    Purpose

    Express tagged H2B

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    miRFP670

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51087

    Purpose

    Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa 1.3 kb

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. AiP12039 - pAAV-3xSP10ins-AiE0354h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2039)

    Plasmid

    #208401

    Purpose

    AiE0354h is an enhancer sequence designed to drive AAV-mediated transgene expression in Vip+ cortical interneurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Lee et al Science. 2023 Oct 13;382(6667):eadf6484.

    Insert

    SYFP2
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    eHGT_354h (enhancer)

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CHOP promoter (-649/+136) pmCherry-1

    Plasmid

    #36035

    Depositor

    Quan Lu

    Article

    Oh et al J Biol Chem. 2012 Feb 17;287(8):6025-34.

    Insert

    CHOP promoter (-647 to +136) (DDIT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HER2 WT

    Plasmid

    #16257

    Depositor

    Mien-Chie Hung

    Article

    Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

    Insert

    HER2 (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LeGO-iG2

    Plasmid

    #27341

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, IRES-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge