165,839 results

1. pTP943

   Plasmid

   #104157

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fab specific nanobody TP886
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-dCas9-p300 Core

   Plasmid

   #61357

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. INCTbiosyn-p35S(rc)2_4_5-GFP

   Plasmid

   #127520

   Purpose

   Plasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRS-LacI-GFP

   Plasmid

   #220198

   Purpose

   mini-Tn7 GFP expression vector - LacI/pLac promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   monomeric superfolder green fluorescent protein
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   monomeric superfolder green fluorescent protein

   Promoter

   LacI/pLac

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-sfGFP

   Plasmid

   #85492

   Purpose

   pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

   Plasmid

   #223712

   Purpose

   Expresses autophagy flux LC3B fluorescence reporter

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   LC3B (MAP1LC3B Human, Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   mCherry::EGFP::LC3B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFLP-hyg

   Plasmid

   #87831

   Purpose

   Expresses FLP recombinase with hyg selection marker

   Depositor

   Pep Charusanti

   Article

   Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

   Insert

   hyg
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   None

   Promoter

   heat-shock inducible

   Available Since

   Aug. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mc2 mNeon

   Plasmid

   #206218

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOpen-P45

   Plasmid

   #165570

   Purpose

   Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   P45
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA-DEST47 PINK1 C-GFP

    Plasmid

    #13316

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET16B.Pfu

    Plasmid

    #12509

    Purpose

    Bacterial expression of Pfu DNA polymerase

    Depositor

    Sung-Hou Kim

    Article

    Goldman et al Acta Crystallogr D Biol Crystallogr. 199

    Insert

    Pfu DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y218H, Q462R, F533L, K559I and V683A (mutations w…

    Promoter

    Available Since

    Sept. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDESTmycDICER

    Plasmid

    #19873

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    dicer 1, ribonuclease type III (DICER1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. PP7_mCherry

    Plasmid

    #61763

    Purpose

    Expresses PP7 coat protein fused to mCherry

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    PP7 coat protein fused to mCherry
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mBFP-APP-mGFP

    Plasmid

    #196694

    Purpose

    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mEGFP and mtagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57.1-DUX4-CA

    Plasmid

    #99281

    Purpose

    All-in-one lentivector with tet-inducible DUX4 (codon sequences altered)

    Depositor

    Stephen Tapscott

    Article

    Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

    Insert

    DUX4 (codon altered) (DUX4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon altered

    Promoter

    TRE

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3 Promoter Vector

    Plasmid

    #212939

    Purpose

    Control vector with constitutive firefly luciferase expression

    Depositor

    Debrya Groskreutz

    Insert

    luc+
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    WT

    Promoter

    SV40

    Available Since

    Jan. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. MSCV-Cre

    Plasmid

    #24064

    Purpose

    Retroviral coexpression of Cre and EGFP

    Depositor

    Dan Littman

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MESV 5' LTR

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. Mouse Metabolic Gene CRISPRa sgRNA library

    Pooled Library

    #187081

    Purpose

    Lentiviral CRISPRa library targeting mouse metabolic genes for activation of gene expression

    Depositor

    Kivanc Birsoy

    Article

    Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    Lentiviral

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

19. mCherry-APP-mGFP

    Plasmid

    #196704

    Purpose

    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mCherry and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTALYM4SpMi-05

    Plasmid

    #47880

    Purpose

    TALE-mRuby2 against Mus spretus/musculus minor satellite sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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