A Highly Versatile Expression System for the Production of Multiply Phosphorylated Proteins.
Zhu P, Gafken PR, Mehl RA, Cooley RB
ACS Chem Biol. 2019 Jul 19;14(7):1564-1572. doi: 10.1021/acschembio.9b00307. Epub 2019 Jun 17. PubMed Article
Zhu P, Gafken PR, Mehl RA, Cooley RB
ACS Chem Biol. 2019 Jul 19;14(7):1564-1572. doi: 10.1021/acschembio.9b00307. Epub 2019 Jun 17. PubMed Article
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174075 | pRBC-sfGFP wild type | Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter |
| 174076 | pRBC-sfGFP-150TAG | Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter, |
| 174077 | pRBC-sfGFP-134/150TAG | Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoter |
| 197655 | B95(DE3) ΔA ΔfabR ΔserB | This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein. |