163,882 results

1. pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)

   Plasmid

   #160566

   Purpose

   GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   Multiplexing Edit (E3-E4-En-1)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-Septin 11 isoform II

   Plasmid

   #120318

   Purpose

   Septin 11 isoform II expression (mCherry-tag at the N-terminus)

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 (Sept11 Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSP64T Activin betaB (DM#108)

   Plasmid

   #15045

   Depositor

   Douglas Melton

   Article

   Thomsen et al Cell. 1990 Nov 2. 63(3):485-93.

   Insert

   Activin Beta b (inhbb Frog)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-Neo-Bam APC

   Plasmid

   #16507

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCXLE-hOCT3/4

   Plasmid

   #27076

   Purpose

   Non-integrating (episomal) expression of human OCT3/4

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   OCT3/4 (POU5F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mouse Brie kinome pooled library, guides 1-4 in lentiGuide-Puro backbone

   Pooled Library

   #75316

   Purpose

   Mouse sgRNA library in backbone lentiGuide-Puro targeting 713 kinase genes and containing 2,852 unique sgRNAs along with 100 non-targeting controls

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-hSyn-DIO-EGFP

   Plasmid

   #50457

   Purpose

   Double floxed EGFP under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, and AAV8

   Insert

   EGFP
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV CreERT2 puro

   Plasmid

   #22776

   Purpose

   Retroviral expression of tamoxifen-inducible Cre-ERT2

   Depositor

   Tyler Jacks

   Article

   Kumar et al Genes Dev. 2009 Dec 1;23(23):2700-4. doi

   Insert

   CreERT2
   

   Use

   Cre/Lox and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Jan. 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVSV-G

   Plasmid

   #138479

   Purpose

   Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   VSV-G envelop protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDZ2087

    Plasmid

    #92414

    Purpose

    Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

    Insert

    TEV protease
    

    Use

    Tags

    MBP (self cleaving)

    Expression

    Bacterial

    Mutation

    L56V/S135G/S219V

    Promoter

    tac

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK122 pDESTattB_exorh:mCherry

    Plasmid

    #213511

    Purpose

    attB-containing pDEST plasmid, for use in Multisite Gateway reaction

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-dLight1.1 (AAV5)

    Viral Prep

    #111067-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CAG-dLight1.1 (#111067). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.1 plasmid DNA. CAG-driven expression of dLight1.1 dopamine sensor. This sensor is recommended for photometry in vivo. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    Tags

    None

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

13. TurboID-His6_pET21a

    Plasmid

    #107177

    Purpose

    expresses His6-tagged TurboID in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. HaloTag-bActinCDS-bActinUTR-MS2V5

    Plasmid

    #102718

    Purpose

    Halo-Actin reporter for detection of both mRNA and protein

    Depositor

    Robert Singer

    Article

    Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    HaloTag-bActinCDS-bActin3'UTR-MS2V5
    

    Use

    Lentiviral

    Tags

    HaloTag and MS2V5

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. LiOn-CAG∞GFP

    Plasmid

    #154015

    Purpose

    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-GFP

    Plasmid

    #16664

    Depositor

    Fred Gage

    Article

    Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLIK_hygro_3xflag_NRF2(RR)

    Plasmid

    #136535

    Purpose

    Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    This plasmid is developed by mutating 3 base pair…

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCBL101-RUBY

    Plasmid

    #199723

    Purpose

    Expresses betalain biosynthesis genes under CaMV 35S promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    35S-RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-Q74

    Plasmid

    #40262

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. PGK-AAVS1ZFNR

    Plasmid

    #60915

    Purpose

    encodes zinc finger nuclease specific to AAVS1 locus (right)

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    AAVS1-ZFNR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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