Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system.
Szczesny RJ, Kowalska K, Klosowska-Kosicka K, Chlebowski A, Owczarek EP, Warkocki Z, Kulinski TM, Adamska D, Affek K, Jedroszkowiak A, Kotrys AV, Tomecki R, Krawczyk PS, Borowski LS, Dziembowski A
PLoS One. 2018 Mar 28;13(3):e0194887. doi: 10.1371/journal.pone.0194887. eCollection 2018.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
105767pKK-NoTagControl vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.
Loading...
105768pKK-FLAG-TEVExpression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.
Loading...
105769pKK-HA-TEVExpression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.
Loading...
105770pKK-FLAG-BirA-TEVExpression of your protein of interest in fusion with FLAG-BirA. FLAG-tagged at the N-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).
Loading...
105771pKK-MBP-TEVExpression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
Loading...
105772pKK-mTagBFP-TEVExpression of your protein of interest in fusion with blue fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.
Loading...
105773pKK-mCerulean-TEVExpression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).
Loading...
105774pKK-mVenus-TEVExpression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
Loading...
105775pKK-mAmber-TEVExpression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the N-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).
Loading...
105776pKK-EGFP-TEV Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)
Loading...
105777pKK-mEGFP-TEVExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).
Loading...
105778pKK-mClover3-TEVExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).
Loading...
105779pKK-mCherry-TEVExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.
Loading...
105780pKK-mRuby3-TEVExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.
Loading...
105781pKK-mCardinal-TEVExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.
Loading...
105782pKK-CyOFP1-TEVExpression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
Loading...
105783pKK-TEV-FLAGExpression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.
Loading...
105784pKK-TEV-3XFLAGExpression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.
Loading...
105785pKK-TEV-HAExpression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.
Loading...
105786pKK-TEV-BirA-FLAGExpression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).
Loading...
105787pKK-TEV-MBPExpression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
Loading...
105788pKK-TEV-ProteinAExpression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.
Loading...
105789pKK-TEV-mTagBFPExpression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.
Loading...
105790pKK-TEV-mCeruleanExpression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).
Loading...
105791pKK-TEV-mVenusExpression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
Loading...
105792pKK-TEV-mAmberExpression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the C-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).
Loading...
105793pKK-TEV-EGFPExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)
Loading...
105794pKK-TEV-mEGFPExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).
Loading...
105795pKK-TEV-mClover3Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).
Loading...
105796pKK-TEV-mCherryExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.
Loading...
105797pKK-TEV-mRuby3Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.
Loading...
105798pKK-TEV-mCardinalExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.
Loading...
105799pKK-TEV-CyOFP1Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
Loading...
105800pKK-BI16-TEV-mCherryConcomitant expression of two proteins. One protein is expressed with mCherry fusion tag (at the C-terminus), cleavable by TEV protease; second tag introduced by user.
Loading...
105801pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.
Loading...
105802pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.
Loading...
105803pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.
Loading...
105804pKK-BiFC-VenusConcomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.
Loading...
105805pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-VenusConcomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.
Loading...
105806pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-AmberConcomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.
Loading...
105807pKK-RNAi-nucEGFPmiR-FLAG-TEVFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal FLAG tag.
Loading...
105808pKK-RNAi-nucEGFPmiR-mCherry-TEVFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal mCherry.
Loading...
105809pKK-RNAi-nucCHERRYmiR-FLAG-TEVFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.
Loading...
105810pKK-RNAi-nucCHERRYmiR-EGFP-TEVFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.
Loading...
105811pKK-RNAi-nucEGFPmiR-TEV-FLAGFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal FLAG.
Loading...
105812pKK-RNAi-nucEGFPmiR-TEV-mCherryFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.
Loading...
105813pKK-RNAi-nucCHERRYmiR-TEV-FLAGFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.
Loading...
105814pKK-RNAi-nucCHERRYmiR-TEV-EGFPFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.
Loading...