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(Plasmid #105806)


Item Catalog # Description Quantity Price (USD)
Plasmid 105806 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    BI-16 (constructed based on pcDNA5/FRT/TO from Invitrogen)
  • Backbone manufacturer
    Sammarco & Grabczyk (PMID: 16212928)
  • Vector type
    Mammalian Expression ; Flp-In competent
  • Selectable markers
  • Tag / Fusion Protein
    • ORF1: 3C-Cerulean; ORF2: TEV-Amber (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Tag / Fusion Protein
    • ORF1: 3C-Cerulean; ORF2: TEV-Amber (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector is a member of the pKK-FRET series which belongs to the pKK vector family. It enables concomitant expression of two genes, which transcription is driven by a tetracycline responsive bidirectional CMV promoter. This vector is a derivate of the BI16 plasmid (PMID: 16212928) and is suitable for stable cell line generation using the Flp-In system (see Szczesny RJ et al. for detailed description and protocols; bioRxiv, doi: Negative control for FRET experiments. One protein expressed with Cerulean whereas second protein expressed with non-fluorescent Amber fusion tag. Amber is a mutant of Venus (Y67C) that retains the tertiary structure but has no fluorescent properties and does not absorb light. A no-FRET sample in which both putative interacting proteins are tagged can be superior to a donor-only sample (PMID: 17040988, 22264545). Amber coding sequence was cloned from Addgene ID: 27798.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber was a gift from Andrzej Dziembowski (Addgene plasmid # 105806 ; ; RRID:Addgene_105806)
  • For your References section:

    Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system. Szczesny RJ, Kowalska K, Klosowska-Kosicka K, Chlebowski A, Owczarek EP, Warkocki Z, Kulinski TM, Adamska D, Affek K, Jedroszkowiak A, Kotrys AV, Tomecki R, Krawczyk PS, Borowski LS, Dziembowski A. PLoS One. 2018 Mar 28;13(3):e0194887. doi: 10.1371/journal.pone.0194887. eCollection 2018. 10.1371/journal.pone.0194887 PubMed 29590189