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Bob Goldstein Lab Plasmids


The Bob Goldstein Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD, Reiner DJ, Goldstein B Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette. Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B Genetics. 2015 Jun 3. pii: genetics.115.178335.
FP-SEC vectors for homologous recombination in C. elegans (unpublished)
Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system (unpublished)
Unpublished SapTrap/SEC plasmids (unpublished)
ID Kit Hidden Extra Search Info  
1000000109SapTrap-SEC kit for C. elegans genome engineering Add to Cart
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
47549pDD162 (Peft-3::Cas9 + Empty sgRNA)Cas9 (Synthetic), Empty sgRNA (Other)Worm Expression, CRISPREngineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641. Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome. pCFJ601 Add to Cart
47550pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)Cas9 (Synthetic), ttTi5605 sgRNA (Other)Worm Expression, CRISPREngineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641. Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele pCFJ601 Add to Cart
47551pDD104 (Peft-3::Cre)Cre RecombinaseWorm Expression, Cre/LoxEngineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641. Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline pCFJ212 Add to Cart
66823pDD282GFP-C1^SEC^3xFlag (Caenorhabditis elegans)Worm Expression, Cre/Lox, CRISPRStreamlined Genome Engineering with a Self-Excising Drug Selection Cassette. Genetics. 2015 Jun 3. pii: genetics.115.178335. GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms pUC19 (modified) Add to Cart
66824pDD283YPET-C1^SEC^3xFlag (Caenorhabditis elegans)Worm Expression, Cre/Lox, CRISPRStreamlined Genome Engineering with a Self-Excising Drug Selection Cassette. Genetics. 2015 Jun 3. pii: genetics.115.178335. YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms pUC19 (modified) Add to Cart
66825pDD284TagRFP-T-C1^SEC^3xFlag (Caenorhabditis elegans)Worm Expression, Cre/Lox, CRISPRStreamlined Genome Engineering with a Self-Excising Drug Selection Cassette. Genetics. 2015 Jun 3. pii: genetics.115.178335. TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms pUC19 (modified) Add to Cart
66826pDD285mKate2-C1^SEC^3xFlag (Caenorhabditis elegans)Worm Expression, Cre/Lox, CRISPRStreamlined Genome Engineering with a Self-Excising Drug Selection Cassette. Genetics. 2015 Jun 3. pii: genetics.115.178335. mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms pUC19 (modified) Add to Cart
70684pDD286 (TagRFP^SEC^3xMyc)TagRFP-T-C1^SEC^3xFlag (Caenorhabditis elegans)Worm Expression, Cre/Lox, CRISPR FP-SEC vectors for homologous recombination in C. elegans (unpublished) TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms. pUC19 (modified) Add to Cart
70685pDD287 (mKate2^SEC^3xMycmKate2-C1^SEC^3xFlag (Caenorhabditis elegans)Worm Expression, Cre/Lox, CRISPR FP-SEC vectors for homologous recombination in C. elegans (unpublished) mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms pUC19 (modified) Add to Cart
73343pDD315 (mTurquoise2^SEC^2xHA)mTurquoise2-C1^SEC^3xFlag (Caenorhabditis elegans)Worm Expression, Cre/Lox, CRISPR FP-SEC vectors for homologous recombination in C. elegans (unpublished) mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms pUC19 (modified) Add to Cart
73344pDD317 (Pmyo-2::GFP + SEC)Pmyo-2::GFP + SEC (Caenorhabditis elegans)Worm Expression, Cre/Lox, CRISPR FP-SEC vectors for homologous recombination in C. elegans (unpublished) SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms pUC19 (modified) Add to Cart
73510JH01pmex-5::GFPo::PH::tbb-2 (Caenorhabditis elegans) Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system (unpublished) GFPo_PH pCFJ150 - contains C. elegans sequence optimized GFP modified pCFJ150 Add to Cart
73511JH02pmex-5::mCherryo::PH::tbb-2 (Caenorhabditis elegans) Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system (unpublished) mCherryo_PH pCFJ150 - contians C. elegans sequence optimized mCherry modified pCFJ150 Add to Cart
73512JH03pmex-5::mKate2::PH::tbb-2 (Caenorhabditis elegans) Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system (unpublished) mKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2 modified pCFJ150 Add to Cart
73513JH04pmex-5::mRuby2::PH::tbb-2 (Caenorhabditis elegans) Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system (unpublished) mRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2 modified pCFJ150 Add to Cart
73516JH05pmex-5::mPYet::PH::tbb-2 (Caenorhabditis elegans) Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system (unpublished) mYPet_PH pCFJ150 - contain C. elegans codon optimized mYPet modified pCFJ150 Add to Cart
73517CH00Xpmex-5::TagRFP-T::PH::tbb-2 (Caenorhabditis elegans) Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system (unpublished) TagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-T modified pCFJ150 Add to Cart
91821pDD399Auxin-inducible degron (AID) (Arabidopsis thaliana)Worm Expression, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) AID CT-tag linker donor for the SapTrap cloning system pMLS280 Add to Cart
91822pDD397Auxin-inducible degron (AID) (Arabidopsis thaliana)Worm Expression, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) AID FP-slot donor for the SapTrap cloning system pMLS280 Add to Cart
91823pDD377mTurquoise2-C1 (Synthetic)Worm Expression, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) Codon-optimized mTurquoise2 donor for the SapTrap cloning system pDONR221 Add to Cart
91824pDD372GFP-C1 (Synthetic)Worm Expression, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) Codon-optimized GFP donor for the SapTrap cloning system pDONR221 Add to Cart
91825pDD375mKate2-C1 (Synthetic)Worm Expression, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) Codon-optimized mKate2 donor for the SapTrap cloning system pMLS280 Add to Cart
91826pMS050mScarlet-I-C1 (Synthetic)Worm Expression, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) Codon-optimized mScarlet-I donor for the SapTrap cloning system pDONR221 Add to Cart
91827pDD378HaloTag-C1 (Synthetic)Worm Expression, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) Codon-optimized HaloTag donor for the SapTrap cloning system pDONR221 Add to Cart
91828pDD401Pmyo-2::GFP (Caenorhabditis elegans)Worm Expression, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) Pmyo-2::GFP FP-slot donor for the SapTrap cloning system pDONR221 Add to Cart
91829pDD363LoxP-SEC-LoxP (Synthetic)Worm Expression, Cre/Lox, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) LoxP-flanked SEC donor for the SapTrap cloning system pDONR221 Add to Cart
91830pDD382Lox2272-SEC-Lox2272 (Synthetic)Worm Expression, Cre/Lox, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) Lox2272-flanked SEC donor for the SapTrap cloning system pDONR221 Add to Cart
91831pDD364Lox511I-SEC-Lox511I (Synthetic)Worm Expression, Cre/Lox, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) Lox511I-flanked SEC donor for the SapTrap cloning system pDONR221 Add to Cart
91832pDD398Auxin-inducible degron (AID) (Arabidopsis thaliana)Worm Expression, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) AID NT-tag linker donor for the SapTrap cloning system pMLS280 Add to Cart
91833pDD121Cas9 (Synthetic)Worm Expression, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) Cas9 expression in C. elegans pCFJ601 Add to Cart
91834pDD379Worm Expression, CRISPR Unpublished SapTrap/SEC plasmids (unpublished) SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold pMLS256 Add to Cart