PurposeYPET^SEC^3xFlag vector with ccdB markers for cloning homology arms
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66824||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepUC19 (modified)
- Backbone size w/o insert (bp) 2600
- Total vector size (bp) 10500
Modifications to backboneAddition of ccdB markers to facilitate homology arm cloning
Vector typeWorm Expression, Cre/Lox, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Turbo
Copy numberHigh Copy
SpeciesC. elegans (nematode), Synthetic
Insert Size (bp)6600
/ Fusion Proteins
- C. elegans codon-optimized YPET
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 Forward (tgtaaaacgacggccagt)
- 3′ sequencing primer M13 Reverse (caggaaacagctatgaccatg) (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDD283 was a gift from Bob Goldstein (Addgene plasmid # 66824 ; http://n2t.net/addgene:66824 ; RRID:Addgene_66824)
For your References section:Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette. Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B. Genetics. 2015 Jun 3. pii: genetics.115.178335. 10.1534/genetics.115.178335 PubMed 26044593