SAC1 degrades its lipid substrate PtdIns4P in the endoplasmic reticulum to maintain a steep chemical gradient with donor membranes.
Zewe JP, Wills RC, Sangappa S, Goulden BD, Hammond GR
Elife. 2018 Feb 20;7. pii: 35588. doi: 10.7554/eLife.35588. PubMed
Zewe JP, Wills RC, Sangappa S, Goulden BD, Hammond GR
Elife. 2018 Feb 20;7. pii: 35588. doi: 10.7554/eLife.35588. PubMed
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 108121 | NES-EGFP-P4Mx1 | PI4P biosensor |
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| 108122 | FKBP-mCherry | Recruitable fluorophore |
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| 108123 | SAC1deltaTMD-FKBP-mCherry | Recruitable cytosolic SAC1 |
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| 108124 | SAC1C389SdeltaTMD-FKBP-mCherry | Recruitable cytosolic inactive SAC1 |
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| 108125 | iRFP-Sec61beta | ER marker |
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| 108126 | mCherry-VAPB | ER marker |
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| 108127 | mCherry-FKBP-SAC1 | Recruitable SAC1 |
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| 108128 | mCherry-FKBP-SAC1C389S | Recruitable inactive SAC1 |
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| 108129 | mCherry-SAC1-FKBP | Recruitable SAC1 |
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| 108130 | mCherry-SAC1C389S-FKBP | Recruitable inactive SAC1 |
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| 108131 | SAC1delta452-587-FKBP-mCherry | Recruitable inactive SAC1 (linker deletion) |
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| 108132 | mCherry-FKBP-SAC1deltaTMD | Recruitable cytosolic SAC1 |
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| 108133 | mCherry-FKBP-SAC1-HLx2 | Recruitable SAC1 with extended linker |
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| 108134 | mCherry-FKBP-SAC1-HLx4 | Recruitable SAC1 with extended linker |
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| 108135 | mCherry-FKBP-SAC1-HLx6 | Recruitable SAC1 with extended linker |
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| 108136 | mCherry-FKBP-SAC1-HLx8 | Recruitable SAC1 with extended linker |
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| 108137 | mCherry-FKBP-SAC1-HLx10 | Recruitable SAC1 with extended linker |
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| 108138 | mCherry-FKBP-SAC1C389S-HLx8 | Recruitable inactive SAC1 with extended linker |
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| 108139 | mCherry-SAC1-HLx8 | SAC1 with extended linker |
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| 108140 | mCherry-SAC1C389S-HLx8 | Inactive SAC1 with extended linker |
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| 108141 | mTagBFP2-FKBP-CYB5Atail | For recruitment of ER |
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| 108142 | NES-mTagBFP2-P4Mx1 | PI4P biosensor |
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| 108143 | NES-mCherry-P4Mx1 | PI4P biosensor |
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| 108144 | NES-iRFP-P4Mx1 | PI4P biosensor |
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