Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

SAC1 degrades its lipid substrate PtdIns4P in the endoplasmic reticulum to maintain a steep chemical gradient with donor membranes.
Zewe JP, Wills RC, Sangappa S, Goulden BD, Hammond GR
Elife. 2018 Feb 20;7. pii: 35588. doi: 10.7554/eLife.35588.

Plasmids from Article

ID Plasmid Purpose
108121NES-EGFP-P4Mx1PI4P biosensor
108122FKBP-mCherryRecruitable fluorophore
108123SAC1deltaTMD-FKBP-mCherryRecruitable cytosolic SAC1
108124SAC1C389SdeltaTMD-FKBP-mCherryRecruitable cytosolic inactive SAC1
108125iRFP-Sec61betaER marker
108126mCherry-VAPBER marker
108127mCherry-FKBP-SAC1Recruitable SAC1
108128mCherry-FKBP-SAC1C389SRecruitable inactive SAC1
108129mCherry-SAC1-FKBPRecruitable SAC1
108130mCherry-SAC1C389S-FKBPRecruitable inactive SAC1
108131SAC1delta452-587-FKBP-mCherryRecruitable inactive SAC1 (linker deletion)
108132mCherry-FKBP-SAC1deltaTMDRecruitable cytosolic SAC1
108133mCherry-FKBP-SAC1-HLx2Recruitable SAC1 with extended linker
108134mCherry-FKBP-SAC1-HLx4Recruitable SAC1 with extended linker
108135mCherry-FKBP-SAC1-HLx6Recruitable SAC1 with extended linker
108136mCherry-FKBP-SAC1-HLx8Recruitable SAC1 with extended linker
108137mCherry-FKBP-SAC1-HLx10Recruitable SAC1 with extended linker
108138mCherry-FKBP-SAC1C389S-HLx8Recruitable inactive SAC1 with extended linker
108139mCherry-SAC1-HLx8SAC1 with extended linker
108140mCherry-SAC1C389S-HLx8Inactive SAC1 with extended linker
108141mTagBFP2-FKBP-CYB5AtailFor recruitment of ER
108142NES-mTagBFP2-P4Mx1PI4P biosensor
108143NES-mCherry-P4Mx1PI4P biosensor
108144NES-iRFP-P4Mx1PI4P biosensor

Antibodies from Article