|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||108129||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneSACM1L (a.k.a. SAC1)
- Promoter CMV
- Cloning method Gibson Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note: The Sac1 insert contains a Y434F mutation compared to the closest reference sequence (NP_054735.3). The depositor has noted that this is a common point mutation that does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mCherry-SAC1-FKBP was a gift from Gerry Hammond (Addgene plasmid # 108129 ; http://n2t.net/addgene:108129 ; RRID:Addgene_108129)
For your References section:SAC1 degrades its lipid substrate PtdIns4P in the endoplasmic reticulum to maintain a steep chemical gradient with donor membranes. Zewe JP, Wills RC, Sangappa S, Goulden BD, Hammond GR. Elife. 2018 Feb 20;7. pii: 35588. doi: 10.7554/eLife.35588. 35588 [pii] PubMed 29461204