pGWB-cLUC
(Plasmid #174051)

• Purpose: (Empty Backbone) Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay
• Depositing Lab: Alberto Macho
• Publication: Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933. doi: 10.1371/journal.ppat.1008933. eCollection 2020 Sep.

Backbone

• Vector backbone: pCAMBIA1300
• Backbone size (bp): 12600
• Modifications to backbone: gateway compatible attR1-CmR-ccdB-attR2
• Vector type: Plant Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Resource Information

• Supplemental Documents:
  • pGWB-cLUC sequence.gb
• A portion of this plasmid was derived from a plasmid made by: a gift from Prof. Jian-Min Zhou in Chinese Academy of Science
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants
https://academic.oup.com/plphys/article/146/2/323/6107098

Please note there are two mutations in Hygr-E180D and A95S. Depositor confirms this does not affect plasmid function.