Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10914||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneE2F4 (a.k.a. E2F-4)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-E2F4 was a gift from Bob Weinberg (Addgene plasmid # 10914 ; http://n2t.net/addgene:10914 ; RRID:Addgene_10914)
For your References section:E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle. Sardet C, Vidal M, Cobrinik D, Geng Y, Onufryk C, Chen A, Weinberg RA. Proc Natl Acad Sci U S A. 1995 Mar 14. 92(6):2403-7. 10.1073/pnas.92.6.2403 PubMed 7892279
Map uploaded by the depositor.