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Efficient Immune Cell Genome Engineering with Enhanced CRISPR Editing Tools.
Chan W, Gottschalk RA, Yao Y, Pomerantz JL, Germain RN
Immunohorizons. 2021 Feb 23;5(2):117-132. doi: 10.4049/immunohorizons.2000082. PubMed Article

Plasmids from Article

ID Plasmid Purpose
167928pCW-Cas9-2A-EGFPDoxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.
167929LentiGuide-TagBFP-2A-BSDLentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.
167930LentiGuide-TagRFP-2A-BSDLentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.
167932LentiCRISPRv2RlentiCRISPRv2 variant with mScarlet-I fluorescence marker.
167933LES2A-CRELentiviral CRE expression tracked by mScarlet-I fluorescence marker.
167934LentiSAMPHv2All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Antibodies from Article