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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBABE-puro
- Backbone size w/o insert (bp) 5169
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSir2
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SpeciesH. sapiens (human)
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Insert Size (bp)2500
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Entrez GeneSIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SnaBI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SspI site in Amp gene.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYESir2-puro was a gift from Bob Weinberg (Addgene plasmid # 1769 ; http://n2t.net/addgene:1769 ; RRID:Addgene_1769) -
For your References section:
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. Vaziri H, Dessain SK, Ng Eaton E, Imai SI, Frye RA, Pandita TK, Guarente L, Weinberg RA. Cell 2001 Oct 19;107(2):149-59. 10.1016/S0092-8674(01)00527-X PubMed 11672523
Map uploaded by the depositor.
