|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1770||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Alt namesilent information regulator
SpeciesH. sapiens (human)
Insert Size (bp)2500
MutationH363Y, residue 363 changed from histidine to tyrosine, dominant negative
Entrez GeneSIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SnaBI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
SspI site in Amp gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYESir2HY-puro was a gift from Bob Weinberg (Addgene plasmid # 1770 ; http://n2t.net/addgene:1770 ; RRID:Addgene_1770)
For your References section:hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. Vaziri H, Dessain SK, Ng Eaton E, Imai SI, Frye RA, Pandita TK, Guarente L, Weinberg RA. Cell 2001 Oct 19;107(2):149-59. 10.1016/S0092-8674(01)00527-X PubMed 11672523