-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 10971 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepTA-Luc
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4800
-
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSIRT1 promoter
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)202
-
Entrez GeneSirt1 (a.k.a. AA673258, S, SIR2L1, Si, Sir2, Sir2a, Sir2alpha)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Resource Information
-
Terms and Licenses
-
Industry Terms
- Not Available to Industry
-
Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTA-Luc SIRT1 promoter (-202) was a gift from Toren Finkel (Addgene plasmid # 10971 ; http://n2t.net/addgene:10971 ; RRID:Addgene_10971) -
For your References section:
Nutrient availability regulates SIRT1 through a forkhead-dependent pathway. Nemoto S, Fergusson MM, Finkel T. Science. 2004 Dec 17. 306(5704):2105-8. 10.1126/science.1101731 PubMed 15604409