|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1789||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepGL3 basic
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Alt nameforkhead responsive element
SpeciesH. sapiens (human)
Insert Size (bp)96
Mutation3 copies of FRE
Entrez GeneFOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
See author's map and sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FHRE-Luc was a gift from Michael Greenberg (Addgene plasmid # 1789 ; http://n2t.net/addgene:1789 ; RRID:Addgene_1789)
For your References section:Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ, Arden KC, Blenis J, Greenberg ME. Cell 1999 Mar 19;96(6):857-68. 10.1016/S0092-8674(00)80595-4 PubMed 10102273