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A photoactivatable Cre-loxP recombination system for optogenetic genome engineering.
Kawano F, Okazaki R, Yazawa M, Sato M
Nat Chem Biol. 2016 Dec;12(12):1059-1064. doi: 10.1038/nchembio.2205. Epub 2016 Oct 10. PubMed Article

Plasmids from Article

ID Plasmid Purpose
122960pcDNA3.1_PA-CrePhotoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)
122961pcDNA3.1_PA-Cre-Y324FCatalytically inactive mutant of PA-Cre
122962pcDNA3.1_Floxed-STOP FlucFirefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reaction
122963pcDNA3.1_Floxed-STOP mCherrymCherry-based fluorescent reporter for Cre-loxP DNA recombination reaction

Antibodies from Article