|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11768||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer LucNrev (Common Sequencing Primers)
Contains three copies of the ARE (activin response element) from the Xenopus Mix.2 promoter linked to a basic TATA box and a luciferase reporter gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pARE-lux was a gift from Joan Massague & Jeff Wrana (Addgene plasmid # 11768 ; http://n2t.net/addgene:11768 ; RRID:Addgene_11768)
For your References section:TGF beta signals through a heteromeric protein kinase receptor complex. Wrana JL, Attisano L, Carcamo J, Zentella A, Doody J, Laiho M, Wang XF, Massague J. Cell. 1992 Dec 11. 71(6):1003-14. 10.1016/0092-8674(92)90395-S PubMed 1333888