p3TP-lux
(Plasmid #11767)

• Depositing Labs: Joan Massague, Jeff Wrana
• Publication: Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Backbone

• Vector backbone: pGL2-Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3TP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LucNrev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was constructed at the Massague lab at MSKCC. Please acknowledge both the Wrana and Massague labs in your publication.
• Articles Citing this Plasmid:
  • 22 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Industry MTA
  • Luciferase Limited Use Label License for Distribution to Industry

Depositor Comments

A luciferase reporter gene with the 3TP
promoter that contains three consecutive TPA response elements (TREs) and a portion of the plasminogen activator
inhibitor 1 (PAI-1) promoter region.